Mass spectrometric quantification of the mu opioid receptor agonist Tyr-D-Arg-Phe-Lys-NH2 (DALDA) in high-performance liquid chromatography-purified ovine plasma

被引:13
作者
Grigoriants, OO
Tseng, JL
Becklin, RR
Desiderio, DM
机构
[1] UNIV TENNESSEE,CHARLES B STOUT NEUROSCI MASS SPECTROMETRY LAB,MEMPHIS,TN 38163
[2] UNIV TENNESSEE,DEPT NEUROL,MEMPHIS,TN 38163
[3] UNIV TENNESSEE,DEPT BIOCHEM,MEMPHIS,TN 38163
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 695卷 / 02期
关键词
DALDA; Tyr-D-Arg-Phe-Lys-NH2; opioids; peptides;
D O I
10.1016/S0378-4347(97)00182-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The mu opioid receptor agonist Tyr-D-Arg-Phe-Lys-Amide (D-Arg(2)-Lys(4)-Dermorphin(1-4)amide=DALDA) was infused continuously for 2 h into sheep. The presence of DALDA in ovine plasma was determined by reversed-phase highperformance liquid chromatography (RP-HPLC) and mass spectrometry (MS) in plasma samples that were obtained at different times during and following that infusion. A stable isotope-incorporated internal standard, deuterated DALDA (d(5)-DALDA), was used for the MS quantification of DALDA via the protonated molecule ion, (M+H)(+), of DALDA and of d(5)-DALDA. Time-course data (mu g DALDA ml(-1) plasma vs. time) were obtained. Tandem MS (MS-MS) provided the product-ion spectrum of the (M+H)(+) ion of DALDA in one of the samples to confirm the amino acid sequence of DALDA.
引用
收藏
页码:287 / 298
页数:12
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