Construction of an similar to 2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2

被引：13

作者：

Wang, ML

Huang, L

BongardPierce, DK

Belmonte, S

Zachgo, EA

Morris, JW

Dolan, M

Goodman, HM

机构：

[1] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02114

[2] MASSACHUSETTS GEN HOSP,DEPT BIOL MOL,BOSTON,MA 02114

来源：

PLANT JOURNAL | 1997年 / 12卷 / 03期

关键词：

D O I：

10.1046/j.1365-313X.1997.00711.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A method for construction of bacterial artificial chromosome (BAG) contigs from a yeast artifical chromosome (YAC) physical map is described. An similar to 2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing.

引用

收藏

页码：711 / 730

页数：20

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