A role of insulin-like growth factor I for follicle-stimulating hormone receptor expression in rat granulosa cells

被引:44
作者
Minegishi, T [1 ]
Hirakawa, T
Kishi, H
Abe, K
Abe, Y
Mizutani, T
Miyamoto, K
机构
[1] Gunma Univ, Sch Med, Dept Obstet & Gynecol, Maebashi, Gumma 3718511, Japan
[2] Fukui Med Univ, Dept Biochem, Fukui 9101193, Japan
关键词
D O I
10.1095/biolreprod62.2.325
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The present study was undertaken to identify the mechanisms underlying the effect of insulin-like growth factor I (ICF-I) on FSH receptor (FSHR) in rat granulosa cells. Treatment with FSH produced a substantial increase in FSHR mRNA level, as was expected, while concurrent treatment with increasing concentrations of ICF-I brought about dose-dependent increases in FSH-induced FSHR mRNA, with a maximal response 2.8-fold greater than that induced by FSH alone. ICF-I, either alone or in combination with FSH, did not affect intracellular cAMP levels, whereas it enhanced the effect of 8-bromo (Br)-cAMP on FSHR mRNA production. Taken together, these findings suggest that the ability of ICF-I to enhance FSH action concerning the induction of FSHR is exerted at sites distal to cAMP generation. We then investigated whether the effect of IGF-I and FSH on FSHR mRNA levels was the result of increased transcription and/or altered mRNA stability. The rates of FSHR mRNA gene transcription, assessed by nuclear run-on transcription assay, were not increased by the addition of ICF-I. On the other hand, the decay curves for the 2.4-kilobase (kb) FSHR mRNA transcript in primary granulosa cells significantly altered the slope of the FSHR mRNA decay curve in the presence of ICF-I and increased the half-life of the FSHR mRNA transcript. These data suggest a possible role for changes in FSHR mRNA stability in the IGF-l-induced regulation of FSHR in rat granulosa cells. Treatment with activin produced a substantial increase in FSHR mRNA level, as was expected, and concurrent treatment with IGF-I did not affect activin-induced FSHR mRNA. Our data suggest that the ICF-I effect on FSHR expression is related to cAMP production induced by FSH and may maintain FSHR mRNA level because of prolonged FSHR mRNA stability.
引用
收藏
页码:325 / 333
页数:9
相关论文
共 29 条
  • [1] INVIVO REGULATION OF GRANULOSA-CELL SOMATOMEDIN-C INSULIN-LIKE GROWTH FACTOR-I RECEPTORS
    ADASHI, EY
    RESNICK, CE
    HERNANDEZ, ER
    SVOBODA, ME
    VANWYK, JJ
    [J]. ENDOCRINOLOGY, 1988, 122 (04) : 1383 - 1389
  • [2] Effects of an Igf1 gene null mutation on mouse reproduction
    Baker, J
    Hardy, MP
    Zhou, J
    Bondy, C
    Lupu, F
    Bellve, AR
    Efstratiadis, A
    [J]. MOLECULAR ENDOCRINOLOGY, 1996, 10 (07) : 903 - 918
  • [3] CELLULAR-LOCALIZATION AND HORMONAL-REGULATION OF FOLLICLE-STIMULATING-HORMONE AND LUTEINIZING-HORMONE RECEPTOR MESSENGER-RNAS IN THE RAT OVARY
    CAMP, TA
    RAHAL, JO
    MAYO, KE
    [J]. MOLECULAR ENDOCRINOLOGY, 1991, 5 (10) : 1405 - 1417
  • [4] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
    CHOMCZYNSKI, P
    SACCHI, N
    [J]. ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) : 156 - 159
  • [5] Role of E box and initiator region in the expression of the rat follicle-stimulating hormone receptor
    Goetz, TL
    Lloyd, TL
    Griswold, MD
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (52) : 33317 - 33324
  • [6] EFFECTS OF FETAL OR MATERNAL HYPOPHYSECTOMY ON ENDOCRINE ORGANS AND BODY-WEIGHT IN INFANT RHESUS-MONKEYS (MACACA-MULATTA) - WITH PARTICULAR EMPHASIS ON OOGENESIS
    GULYAS, BJ
    HODGEN, GD
    TULLNER, WW
    ROSS, GT
    [J]. BIOLOGY OF REPRODUCTION, 1977, 16 (02) : 216 - 227
  • [7] Identification of a hormonally regulated luteinizing hormone human chorionic gonadotropin receptor mRNA binding protein - Increased mRNA binding during receptor down-regulation
    Kash, JC
    Menon, KMJ
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (17) : 10658 - 10664
  • [8] The effect of activin and FSH on the differentiation of rat granulosa cells
    Kishi, H
    Minegishi, T
    Tano, M
    Kameda, T
    Ibuki, Y
    Miyamoto, K
    [J]. FEBS LETTERS, 1998, 422 (02) : 274 - 278
  • [9] Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility
    Kumar, TR
    Wang, Y
    Lu, NF
    Matzuk, MM
    [J]. NATURE GENETICS, 1997, 15 (02) : 201 - 204
  • [10] GONADOTROPIN-INDUCED UP-REGULATION AND DOWN-REGULATION OF OVARIAN FOLLICLE-STIMULATING-HORMONE (FSH) RECEPTOR GENE-EXPRESSION IN IMMATURE RATS - EFFECTS OF PREGNANT MARES SERUM GONADOTROPIN, HUMAN CHORIONIC-GONADOTROPIN, AND RECOMBINANT FSH
    LAPOLT, PS
    TILLY, JL
    AIHARA, T
    NISHIMORI, K
    HSUEH, AJW
    [J]. ENDOCRINOLOGY, 1992, 130 (03) : 1289 - 1295