Determination of clenbuterol by capillary electrophoresis immunoassay with chemiluminescence detection

被引：66

作者：

Ji, Xinghu

He, Zhike ^{[1
]}

Ai, Xinping

Yang, Hanxi

Xu, Chuanlai

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China

[2] So Yangtze Univ, Sch Food Sci & Technol, Wuxi 214036, Peoples R China

来源：

TALANTA | 2006年 / 70卷 / 02期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

capillary electrophoresis; chemiluminescence; immunoassay; clenbuterol;

D O I：

10.1016/j.talanta.2006.02.053

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A competitive immunoassay for clenbuterol (CLB) based on capillary electrophoresis with chemiluminescence (CL) detection was established. The method was based on the competitive reaction of horseradish peroxidase (HRP)-labeled CLB (CLB-HRP) and free CLB with anti-CLB antiserum. The factors affecting the electrophoresis and CL detection were systematically investigated with HRP as a model sample. Under the optimal conditions, the tracer CLB-HRP and the immunoassay complex were separated, and the linear range and the detection limit (S/N=3) for CLB were 5.0-40 nmol l(-1) and 1.2 nmol l(-1), respectively. The proposed method has been applied satisfactorily in the analysis of urine sample. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：353 / 357

页数：5

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[11] Bioanalysis of beta(2)-agonists by hyphenated chromatographic and mass spectrometric techniques [J].