Induction of apoptosis in equine chorionic gonadotropin (eCG)-primed rat ovaries by anti-eCG antibody

被引:46
作者
Boone, DL
Carnegie, JA
Rippstein, PU
Tsang, BK
机构
[1] UNIV OTTAWA, DEPT OBSTET & GYNAECOL, REPROD BIOL UNIT, OTTAWA, ON K1Y 4E9, CANADA
[2] UNIV OTTAWA, DEPT CELLULAR & MOL MED, OTTAWA, ON K1Y 4E9, CANADA
[3] OTTAWA CIVIC HOSP, LOEB RES INST, OTTAWA, ON K1Y 4E9, CANADA
[4] OTTAWA CIVIC HOSP, DEPT LAB MED, OTTAWA, ON K1Y 4E9, CANADA
[5] AGR & AGRI FOOD CANADA, CTR FOOD & ANIM RES, OTTAWA, ON K1A 0C6, CANADA
关键词
D O I
10.1095/biolreprod57.2.420
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have established a model to examine the early events of apoptosis in small antral follicles in vivo. Immature female rats injected with 15 IU eCG, and subsequently (24 h later) with an anti-eCG antibody to induce gonadotropin withdrawal, displayed a significantly lower ovarian weight and increased incidence of follicular atresia and granulosa cell death, especially in small- to medium-sized follicles. Evidence of apoptosis was apparent in a significantly higher proportion of granulosa cells from antibody-treated rats, which exhibited membrane blebbing, nuclear and cytoplasmic condensation, fragmentation, and phagocytosis. In addition, there was a loss of the regular organization of the lamina densa in the follicular basement membrane. Degradation of DNA was consistently found by 24 h in the antibody-treated group, and ladders could be observed as early as 1 h after treatment. Although cell death was observed after antibody treatment in some larger antral follicles, the occurrence of apoptosis was less frequent. These results demonstrate that gonadotropin withdrawal in vivo rapidly induces apoptosis in small- to medium-sized antral follicles at the critical stage of development when atresia is commonly observed, suggesting that this model is ideal for studying apoptosis in the ovary.
引用
收藏
页码:420 / 427
页数:8
相关论文
共 26 条
  • [11] Hormonal regulation of apoptosis in early antral follicles: Follicle-stimulating hormone as a major survival factor
    Chun, SY
    Eisenhauer, KM
    Minami, S
    Billig, H
    Perlas, E
    Hsueh, AJW
    [J]. ENDOCRINOLOGY, 1996, 137 (04) : 1447 - 1456
  • [12] IMPOSSIBILITY OF ACRIDINE-ORANGE INTERCALATION IN NUCLEAR-DNA OF THE LIVING CELL
    DELIC, J
    COPPEY, J
    MAGDELENAT, H
    COPPEYMOISAN, M
    [J]. EXPERIMENTAL CELL RESEARCH, 1991, 194 (01) : 147 - 153
  • [13] GRANULOSA-CELL FUSION ALLOWS HETEROLOGOUS RECEPTOR STIMULATION OF ADENYLATE-CYCLASE AND PROGESTERONE ACCUMULATION
    FAROOKHI, R
    [J]. ENDOCRINOLOGY, 1982, 110 (03) : 1061 - 1063
  • [14] FAROOKHI R, 1980, DYNAMICS OVARIAN FUN, P13
  • [15] Greenwald G.S., 1988, P387
  • [16] DEVELOPMENT OF FOLLICLES IN THE MAMMALIAN OVARY
    HIRSHFIELD, AN
    [J]. INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1991, 124 : 43 - 101
  • [17] SIZE-FREQUENCY ANALYSIS OF ATRESIA IN CYCLING RATS
    HIRSHFIELD, AN
    [J]. BIOLOGY OF REPRODUCTION, 1988, 38 (05) : 1181 - 1188
  • [18] OVARIAN FOLLICLE ATRESIA - A HORMONALLY CONTROLLED APOPTOTIC PROCESS
    HSUEH, AJW
    BILLIG, H
    TSAFRIRI, A
    [J]. ENDOCRINE REVIEWS, 1994, 15 (06) : 707 - 724
  • [19] BIOCHEMICAL-IDENTIFICATION OF APOPTOSIS (PROGRAMMED CELL-DEATH) IN GRANULOSA-CELLS - EVIDENCE FOR A POTENTIAL MECHANISM UNDERLYING FOLLICULAR ATRESIA
    HUGHES, FM
    GOROSPE, WC
    [J]. ENDOCRINOLOGY, 1991, 129 (05) : 2415 - 2422
  • [20] Early onset of deoxyribonucleic acid fragmentation during atresia of preovulatory ovarian follicles in rats
    Nahum, R
    Beyth, Y
    Chun, SY
    Hsueh, AJW
    Tsafriri, A
    [J]. BIOLOGY OF REPRODUCTION, 1996, 55 (05) : 1075 - 1080