Promotion of nicotine biosynthesis in transgenic tobacco by overexpressing allene oxide cyclase from Hyoscyamus niger

被引：18

作者：

Jiang, Keji ^{[2
,3
]}

Pi, Yan ^{[2
]}

Hou, Rong ^{[2
]}

Jiang, Lili ^{[4
]}

Sun, Xiaofen ^{[2
]}

Tang, Kexuan ^{[1
,2
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Key Lab Agrobiotechnol, Plant Biotechnol Res Ctr, Sch Agr & Biol,Inst Syst Biol,Fudan SJTU Nottingh, Shanghai 200030, Peoples R China

[2] Fudan Univ, State Key Lab Genet Engn, Inst Genet, Sch Life Sci,Fudan SJTU Nottingham Plant Biotechn, Shanghai 200433, Peoples R China

[3] Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

[4] Shanghai Putuo Municipal Ctr Dis Control & Preven, Shanghai 20033, Peoples R China

来源：

PLANTA | 2009年 / 229卷 / 05期

关键词：

Allene oxide cyclase; Hyoscyamus niger; Jasmonate; Metabolic engineering; Metabolite; Nicotiana tabacum; Nicotine; Transformation; WOUND-INDUCED CHANGES; JASMONIC ACID POOLS; METHYL JASMONATE; MOLECULAR-CLONING; HAIRY ROOTS; ALKALOIDS; EXPRESSION; PATHWAY; PLANTS; METABOLISM;

D O I：

10.1007/s00425-009-0896-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plant secondary metabolites are a wide variety of low-molecular weight compounds whose productions are often enhanced in response to both biotic and abiotic stresses. Many of the responses are mediated by a class of hormones, named as jasmonates. In jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most crucial step. Here a heterologous AOC gene from Hyoscyamus niger L. (black henbane), named HnAOC (GenBank accession No. AY708383), was overexpressed in Nicotiana tabacum cv. Petit Havana to investigate the consequence on nicotine content. This study revealed that the transcription of HnAOC in tobacco resulted in overexpression of nicotine biosynthetic pathway genes and higher yield of nicotine, with the maximum of 4.8-fold over control. Therefore, it indicated that without the cost of extrinsic hormones, genetic manipulation of jasmonate biosynthetic pathway genes could be an alternative approach in metabolic engineering for the production of valuable secondary metabolites, which were induced by jasmonates.

引用

页码：1057 / 1063

页数：7

共 32 条

[11] Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosynthesis in tobacco cell cultures [J].