Heteroduplex analysis in medical entomology: A rapid and sensitive sequence-based tool for population and phylogenetic studies

被引:13
作者
Tang, J [1 ]
Unnasch, TR [1 ]
机构
[1] UNIV ALABAMA,DEPT MED,DIV GEOG MED,BIRMINGHAM,AL 35294
来源
PARASITOLOGY TODAY | 1997年 / 13卷 / 07期
基金
美国国家卫生研究院;
关键词
D O I
10.1016/S0169-4758(97)01062-4
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Classical studies on the insect vectors of parasitic diseases have relied on morphological, biochemical and cytological characters to classify vector species. These are often inadequate for a detailed dissection of the dynamics of the parasite-vector relationship. Molecular techniques have the potential to provide move reliable data on various aspects of vector biology, including population genetics, molecular phylogenetics, and molecular differentiation and classification of closely related species. However, most molecular techniques are expensive, cumbersome or difficult to apply to the analysis of a large number of samples. Here, Jianming Tang and Tom Unnasch discuss the practical advantages of heteroduplex analysis for the study of medically important vectors, using the black flies that serve as vectors for the filarial parasite Onchocerca volvulus as an example. This technique is simple, rapid, inexpensive and capable of detecting minor differences among DNA sequences.
引用
收藏
页码:271 / 274
页数:4
相关论文
共 15 条
[1]   BASE-BASE MISMATCHES - THERMODYNAMICS OF DOUBLE HELIX FORMATION FOR DCA3XA3G + DCT3YT3G (X, Y = A,C,G,T) [J].
ABOULELA, F ;
KOH, D ;
TINOCO, I ;
MARTIN, FH .
NUCLEIC ACIDS RESEARCH, 1985, 13 (13) :4811-4824
[2]   GENETIC-RELATIONSHIPS DETERMINED BY A DNA HETERODUPLEX MOBILITY ASSAY - ANALYSIS OF HIV-1 ENV GENES [J].
DELWART, EL ;
SHPAER, EG ;
LOUWAGIE, J ;
MCCUTCHAN, FE ;
GREZ, M ;
RUBSAMENWAIGMANN, H ;
MULLINS, JI .
SCIENCE, 1993, 262 (5137) :1257-1261
[4]  
LI WH, 1991, FUNDAMENTALS MOL EVO, P86
[5]   EVOLUTION, WEIGHTING, AND PHYLOGENETIC UTILITY OF MITOCHONDRIAL GENE-SEQUENCES AND A COMPILATION OF CONSERVED POLYMERASE CHAIN-REACTION PRIMERS [J].
SIMON, C ;
FRATI, F ;
BECKENBACH, A ;
CRESPI, B ;
LIU, H ;
FLOOK, P .
ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA, 1994, 87 (06) :651-701
[6]  
SWOFFORD DL, 1991, PAUP PHYLOGENETIC AN
[7]   THE SIMULIUM-DAMNOSUM SPECIES COMPLEX - PHYLOGENETIC ANALYSIS AND MOLECULAR-IDENTIFICATION BASED UPON MITOCHONDRIALLY ENCODED GENE-SEQUENCES [J].
TANG, J ;
TOE, L ;
BACK, C ;
ZIMMERMAN, PA ;
PRUESS, K ;
UNNASCH, TR .
INSECT MOLECULAR BIOLOGY, 1995, 4 (02) :79-88
[8]   MITOCHONDRIAL ALLELES OF SIMULIUM-DAMNOSUM SENSU-LATO INFECTED WITH ONCHOCERCA-VOLVULUS [J].
TANG, JM ;
TOE, L ;
BACK, C ;
UNNASCH, TR .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1995, 25 (10) :1251-1254
[9]   Genotyping north American black flies by means of mitochondrial ribosomal RNA sequences [J].
Tang, JM ;
Pruess, K ;
Unnasch, TR .
CANADIAN JOURNAL OF ZOOLOGY, 1996, 74 (01) :39-46
[10]  
WANG YH, 1992, J BIOL CHEM, V267, P4911