Senescence marker protein-30 as a novel antiaging molecule

被引：22

作者：

Feng, DY

Kondo, Y

Ishigami, A

Kuramoto, M

Machida, T

Maruyama, N

机构：

[1] Saitama Univ, Fac Sci, Dept Regulat Biol, Urawa, Saitama, Japan

[2] Tokyo Med & Dent Univ, Sch Med, Tokyo 1138549, Japan

来源：

STRATEGIES FOR ENGINEERED NEGLIGIBLE SENESCENCE: WHY GENUINE CONTROL OF AGING MAY BE FORESEEABLE | 2004年 / 1019卷

关键词：

SMP30; DFPase; aging;

D O I：

10.1196/annals.1297.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Senescence marker protein-30 (SMP30), composed of 299 amino acids, has an approximate molecular mass of 32-34 kDa and has a pI 4.9 in charge. The amino acid alignment from various animal species revealed a highly conserved structure. SMP30 has an enzyme activity hydrolyzing sarin, soman, and tabun, known as lethal toxic nerve chemicals. We analyzed the organophosphatase activity of SMP30 using DFP as a substrate. This DFPase activity is revealed in a dose-dependent manner in the presence of magnesium ions. We investigated the intracellular localization of SMP30. It is localized in both the cytoplasm and nucleus. To confirm the presence of SMP30 in the nucleus, we prepared nuclear and cytoplasmic extracts from isolated cultured hepatocytes. Western blotting showed that SMP30 was detected in both extracts. Because the expression is reduced by carbon tetrachloride, one can speculate that the expression is modulated by oxidative stress increased with aging.

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页码：360 / 364

页数：5

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