Spectrally resolved absolute fluorescence cross sections for Bacillus spores

被引:75
作者
Faris, GW
Copeland, RA
Mortelmans, K
Bronk, BV
机构
[1] SRI INT,CELL & MOL BIOL LAB,MENLO PK,CA 94025
[2] EDGEWOOD RES DEV & ENGN CTR,ABERDEEN PROVING GROUND,MD 21010
来源
APPLIED OPTICS | 1997年 / 36卷 / 04期
关键词
D O I
10.1364/AO.36.000958
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Absolute fluorescence cross sections for Bacillus subtilis and B. cereus bacterial spores as both aqueous suspensions and aerosols were measured at a number of excitation wavelengths between 228 and 303 nm. The fluorescence was spectrally resolved at each excitation wavelength. excitation wavelength for spore fluorescence is between 270 and 280 nm for aqueous suspensions is four times larger than for dry aerosols when measured under similar conditions. Measurements on wet aerosols showed an increase in fluorescence cross section over dry aerosols, indicating an enhancement of the fluorescence when the bacterial spores are wet. Mie scattering cross sections at 90 degrees to the direction of the incident radiation and extinction cross sections as a function of wavelength for B. subtilis suspensions and fluorescence cross sections for tryptophan are also reported. (C) 1997 Optical Society of America
引用
收藏
页码:958 / 967
页数:10
相关论文
共 32 条
  • [1] SIMPLE, ONE-STEP FLUOROMETRIC METHOD FOR DETERMINATION OF NANOMOLAR CONCENTRATIONS OF TERBIUM
    BARELA, TD
    SHERRY, AD
    [J]. ANALYTICAL BIOCHEMISTRY, 1976, 71 (02) : 351 - 357
  • [2] BACTERIAL SPORE HEAT-RESISTANCE CORRELATED WITH WATER-CONTENT, WET DENSITY, AND PROTOPLAST SPOROPLAST VOLUME RATIO
    BEAMAN, TC
    GREENAMYRE, JT
    CORNER, TR
    PANKRATZ, HS
    GERHARDT, P
    [J]. JOURNAL OF BACTERIOLOGY, 1982, 150 (02) : 870 - 877
  • [3] BISCHEL WK, 1984, CRDCCR84102 SRI INT
  • [4] BISCHEL WK, 1983, EXCIMER LASERS 1983, P181
  • [5] VARIABILITY OF STEADY-STATE BACTERIAL FLUORESCENCE WITH RESPECT TO GROWTH-CONDITIONS
    BRONK, BV
    REINISCH, L
    [J]. APPLIED SPECTROSCOPY, 1993, 47 (04) : 436 - 440
  • [6] CHEN R F, 1967, Analytical Letters, V1, P35
  • [7] CHEN RF, 1990, PRACTICAL FLUORESCEN, P621
  • [8] IDENTIFICATION OF BACTERIAL PATHOGENS BY LASER-EXCITED FLUORESCENCE
    COBURN, JT
    LYTLE, FE
    HUBER, DM
    [J]. ANALYTICAL CHEMISTRY, 1985, 57 (08) : 1669 - 1673
  • [9] THE STEADY-STATE AND DECAY CHARACTERISTICS OF PRIMARY FLUORESCENCE FROM LIVE BACTERIA
    DALTERIO, RA
    NELSON, WH
    BRITT, D
    SPERRY, JF
    TANGUAY, JF
    SUIB, SL
    [J]. APPLIED SPECTROSCOPY, 1987, 41 (02) : 234 - 241
  • [10] STEADY-STATE AND DECAY CHARACTERISTICS OF PROTEIN TRYPTOPHAN FLUORESCENCE FROM BACTERIA
    DALTERIO, RA
    NELSON, WH
    BRITT, D
    SPERRY, J
    PSARAS, D
    TANGUAY, JF
    SUIB, SL
    [J]. APPLIED SPECTROSCOPY, 1986, 40 (01) : 86 - 90