Spectrally resolved absolute fluorescence cross sections for Bacillus spores
被引:75
作者:
Faris, GW
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机构:SRI INT,CELL & MOL BIOL LAB,MENLO PK,CA 94025
Faris, GW
Copeland, RA
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机构:SRI INT,CELL & MOL BIOL LAB,MENLO PK,CA 94025
Copeland, RA
Mortelmans, K
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机构:SRI INT,CELL & MOL BIOL LAB,MENLO PK,CA 94025
Mortelmans, K
Bronk, BV
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机构:SRI INT,CELL & MOL BIOL LAB,MENLO PK,CA 94025
Bronk, BV
机构:
[1] SRI INT,CELL & MOL BIOL LAB,MENLO PK,CA 94025
[2] EDGEWOOD RES DEV & ENGN CTR,ABERDEEN PROVING GROUND,MD 21010
来源:
APPLIED OPTICS
|
1997年
/
36卷
/
04期
关键词:
D O I:
10.1364/AO.36.000958
中图分类号:
O43 [光学];
学科分类号:
070207 ;
0803 ;
摘要:
Absolute fluorescence cross sections for Bacillus subtilis and B. cereus bacterial spores as both aqueous suspensions and aerosols were measured at a number of excitation wavelengths between 228 and 303 nm. The fluorescence was spectrally resolved at each excitation wavelength. excitation wavelength for spore fluorescence is between 270 and 280 nm for aqueous suspensions is four times larger than for dry aerosols when measured under similar conditions. Measurements on wet aerosols showed an increase in fluorescence cross section over dry aerosols, indicating an enhancement of the fluorescence when the bacterial spores are wet. Mie scattering cross sections at 90 degrees to the direction of the incident radiation and extinction cross sections as a function of wavelength for B. subtilis suspensions and fluorescence cross sections for tryptophan are also reported. (C) 1997 Optical Society of America