Unusually high reactivity of apolipoprotein B-100 among proteins to radical reactions induced in human plasma

被引：18

作者：

Hashimoto, R ^{[1
]}

Narita, S ^{[1
]}

Yamada, Y ^{[1
]}

Tanaka, K ^{[1
]}

Kojo, S ^{[1
]}

机构：

[1] Nara Womens Univ, Dept Food Sci & Nutr, Nara 6308506, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2000年 / 1483卷 / 02期

关键词：

albumin; apolipoprotein B-100; low density lipoprotein; protein degradation; radical; transferrin;

D O I：

10.1016/S1388-1981(99)00167-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Relative reactivities of proteins to radical reactions caused in human plasma were studied for the first time utilizing an immunoblotting assay. When radical reactions were caused by Cu2+, apolipoprotein B-100 (apoB) underwent extensive fragmentation concurrently with the decrease in cr-tocopherol, while human serum albumin (HSA) and transferrin (TF) were not decreased at all. When radical reactions were initiated by Cu2+ with hydrogen peroxide or 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH), alpha-tocopherol and apoB were also decreased steadily but HSA and TF were not decreased. These observations indicate that apoB is extremely reactive, even comparable to alpha-tocopherol, towards radical reactions. These results also suggest that the radical reaction of apoB is a possible process in vivo and it is involved in atherogenesis along with low density lipoprotein lipid peroxidation, which has been studied extensively. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：236 / 240

页数：5

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