Biosensing, Cytotoxicity, and Cellular Uptake Studies of Surface-Modified Gold Nanorods

被引：121

作者：

Parab, Harshala J. ^{[1
,3
]}

Chen, Hao Ming ^{[1
]}

Lai, Tsung-Ching ^{[4
]}

Huang, Jing Hong ^{[1
]}

Chen, Po Hsun ^{[4
]}

Liu, Ru-Shi ^{[1
]}

Hsiao, Michael ^{[4
]}

Chen, Chung-Hsuan ^{[4
]}

Tsai, Din-Ping ^{[2
]}

Hwu, Yeu-Kuang ^{[3
]}

机构：

[1] Natl Taiwan Univ, Dept Chem, Taipei 106, Taiwan

[2] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan

[3] Acad Sinica, Inst Phys, Taipei 115, Taiwan

[4] Acad Sinica, Genom Res Ctr, Taipei 115, Taiwan

来源：

JOURNAL OF PHYSICAL CHEMISTRY C | 2009年 / 113卷 / 18期

关键词：

HIGH-YIELD SYNTHESIS; PLASMON RESONANCE; METAL NANOPARTICLES; TOXICITY; SHAPE; SIZE; SENSITIVITY; CHEMISTRY; MECHANISM; BIOLOGY;

D O I：

10.1021/jp9000169

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Herein we report biorecognition studies of protein IgG using biocomapatible gold nanorods as molecular probes. Surface modification of cetyltrimethylammonium bromide (CTAB)-stabilized gold nanorods was carried out by using poly(styrenesulfonate) (PSS) to reduce the toxicity of as-synthesized gold nanorods caused by free CTAB. zeta potential analysis confirmed charge reversal on the surface of gold nanorods caused by the PSS coating. Surface plasmon resonance exhibited by gold nanorods has been employed as a tool for analyzing the binding events for biomolecules. TEM results, showing the aggregation of gold nanorods, in addition to the shift in surface plasmon resonance peak in UV-vis absorption measurements, upon the interaction of biomolecules with gold nanorods, confirmed molecular binding. Morphological changes caused by the cellular uptake of gold nanorods before and after PSS modification have been observed. Cell viability studies using gold nanorods were performed to study the cytotoxic effects of these molecular probes.

引用

页码：7574 / 7578

页数：5

共 39 条

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