Kinetic flux profiling of nitrogen assimilation in Escherichia coli

被引：103

作者：

Yuan, Jie

Fowler, William U.

Kimball, Elizabeth

Lu, Wenyun

Rabinowitz, Joshua D. ^{[1
]}

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

[2] Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08544 USA

来源：

NATURE CHEMICAL BIOLOGY | 2006年 / 2卷 / 10期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1038/nchembio816

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.

引用

收藏

页码：529 / 530

页数：2

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