Redistribution of actin, profilin and phosphatidylinositol-4,5-bisphosphate in growing and maturing root hairs

被引:95
作者
Braun, M [1 ]
Baluska, F [1 ]
von Witsch, M [1 ]
Menzel, D [1 ]
机构
[1] Univ Bonn, Bot Inst, D-53115 Bonn, Germany
关键词
actin; cytochalasin D; mastoparan; phosphatidylinositol-4,5-bisphosphate; profilin; tip growth;
D O I
10.1007/s004250050746
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The continuously changing polar cytoplasmic organization during initiation and tip growth of root hairs is reflected by a dynamic redistribution of cytoskeletal elements. The small G-actin binding protein, profilin, which is known to be a widely expressed, potent regulator of actin dynamics, was specifically localized at the tip of root hairs and co-distributed with a diffusely fluorescing apical cap of actin, but not with subapical actin microfilament (MF) bundles. Profilin and actin caps were present exclusively in the bulge of outgrowing root hairs and at the apex of elongating root hairs; both disappeared when tip growth terminated, indicating a tip-growth mechanism that involves profilin-actin interactions for the delivery and localized exocytosis of secretory vesicles. Phosphatidylinositol-4,5-bisphosphate (PIP2), a ligand of profilin, was localized almost exclusively in the bulge and, subsequently, formed a weak tip-to-base gradient in the elongating root hairs. When tip growth was eliminated by the MF-disrupting inhibitor cytochalasin D, the apical profilin and the actin fluorescence were lost. Mastoparan, which is known to affect the PIP2 cycle, probably by stimulating phospholipases, caused the formation of a meshwork of distinct actin MFs replacing the diffuse apical actin cap and, concomittantly, tip growth stopped. This suggests that mastoparan interferes with the PIP2-regulated profilinactin interactions and hence disturbs conditions indispensable for the maintenance of tip growth in root hairs.
引用
收藏
页码:435 / 443
页数:9
相关论文
共 52 条
  • [11] Actin-binding proteins in plant cells
    de Ruijter, NCA
    Emons, AMC
    [J]. PLANT BIOLOGY, 1999, 1 (01) : 26 - 35
  • [12] Phosphatidylinositol 4,5-bisphosphate specifically stimulates PP60(c-src) catalyzed phosphorylation of gelsolin and related actin-binding proteins
    DeCorte, V
    Gettemans, J
    Vandekerckhove, J
    [J]. FEBS LETTERS, 1997, 401 (2-3) : 191 - 196
  • [13] INOSITOL(1,4,5)TRISPHOSPHATE PRODUCTION IN PLANT-CELLS - STIMULATION BY THE VENOM PEPTIDES, MELITTIN AND MASTOPARAN
    DROBAK, BK
    WATKINS, PAC
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 205 (01) : 739 - 745
  • [14] INHIBITION OF PLANT PLASMA-MEMBRANE PHOSPHOINOSITIDE PHOSPHOLIPASE-C BY THE ACTIN-BINDING PROTEIN, PROFILIN
    DROBAK, BK
    WATKINS, PAC
    VALENTA, R
    DOVE, SK
    LLOYD, CW
    STAIGER, CJ
    [J]. PLANT JOURNAL, 1994, 6 (03) : 389 - 400
  • [15] THE CYTOSKELETON AND SECRETORY VESICLES IN ROOT HAIRS OF EQUISETUM AND LIMNOBIUM AND CYTOPLASMIC STREAMING IN ROOT HAIRS OF EQUISETUM
    EMONS, AMC
    [J]. ANNALS OF BOTANY, 1987, 60 (06) : 625 - 632
  • [16] FOCUSING ON UNPOLYMERIZED ACTIN
    FECHHEIMER, M
    ZIGMOND, SH
    [J]. JOURNAL OF CELL BIOLOGY, 1993, 123 (01) : 1 - 5
  • [17] Pollen profilin function depends on interaction with proline-rich motifs
    Gibbon, BC
    Zonia, LE
    Kovar, DR
    Hussey, PJ
    Staiger, CJ
    [J]. PLANT CELL, 1998, 10 (06) : 981 - 993
  • [18] Gibbon BC, 1997, BIOCHEM J, V327, P909
  • [19] INTERACTION OF PLANT PROFILIN WITH MAMMALIAN ACTIN
    GIEHL, K
    VALENTA, R
    ROTHKEGEL, M
    RONSIEK, M
    MANNHERZ, HG
    JOCKUSCH, BM
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 226 (02): : 681 - 689
  • [20] PROTEIN-KINASE C-DEPENDENT PHOSPHORYLATION OF PROFILIN IS SPECIFICALLY STIMULATED BY PHOSPHATIDYLINOSITOL BISPHOSPHATE (PIP2)
    HANSSON, A
    SKOGLUND, G
    LASSING, I
    LINDBERG, U
    INGELMANSUNDBERG, M
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 150 (02) : 526 - 531