Identification of thermoacidophilic bacteria and a new Alicyclobacillus genomic species isolated from acidic environments in Japan

被引:46
作者
Goto, K
Tanimoto, Y
Tamura, T
Mochida, K
Arai, D
Asahara, M
Suzuki, M
Tanaka, H
Inagaki, K
机构
[1] Mitsui Norin Co Ltd, Food Res Labs, Microbiol & Analyt Grp, Shizuoka 4260133, Japan
[2] Okayama Univ, Fac Agr, Dept Bioresources Chem, Okayama 700, Japan
[3] Tokyo Food Techno Co Ltd, Cent Res Labs, Shizuoka, Japan
关键词
identification; Alicyclobacillus; 16S rDNA; HV region; Alicyclobacillus genomic species 2; thermoacidophile; distribution;
D O I
10.1007/s00792-001-0262-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sixty strains of thermoacidophilic bacteria have been isolated from soil and water samples obtained from various acidic environments in Japan. An initial comparative sequence analysis of the hypervariable regions of the 16S rDNA revealed that all strains could be assigned to the Alicyclobacillus acidocaldarius-Alicyclobacillus genomic species 1 group, which could be further subdivided into three clusters (Clusters I-III). On the basis of phenotypic characteristics, chemotaxonomic profiles, and phylogenetic data of six selected strains, five strains were identified as either A. acidocaldarius or Alicyclobacillus genomic species 1; however, one strain (MIH 332) could not be determined to belong to either of these species. 16S rDNA sequence homology values between strain MIH 332 and the reference strains of A. acidocaldarius (ATCC 27009(T)) and Alicyclobacillus genomic species 1 (DSM 11984) were 98.8% and 99.1%, respectively, which were higher than the corresponding similarity between the reference strains (98.4%). On the other hand, DNA-DNA hybridization levels between strain MIH 332 and the reference strains were 39% and 44%, respectively, which were lower than the value between the reference strains (59% or 65%). However, the phenotype of strain MIH 332 was also similar to those of the reference strains, and a typical phenotype could not be found for the strain, thus indicating that the strain may be a new genomic species of A. acidocaldarius, for which the name Alicyclobacillus genomic species 2 is tentatively proposed. The results of this study suggest that A. acidocaldarius and its related species are widely distributed in acidic environments in Japan, with slight regional variations in morphological and genotypic characteristics.
引用
收藏
页码:333 / 340
页数:8
相关论文
共 33 条
[1]   Alicyclobacillus hesperidum sp nov and a related genomic species from solfataric soils of Sao Miguel in the Azores [J].
Albuquerque, L ;
Rainey, FA ;
Chung, AP ;
Sunna, A ;
Nobre, MF ;
Grote, R ;
Antranikian, G ;
da Costa, MS .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :451-457
[2]   A microbiological survey of Montserrat Island hydrothermal biotopes [J].
Atkinson, T ;
Gairns, S ;
Cowan, DA ;
Danson, MJ ;
Hough, DW ;
Johnson, DB ;
Norris, PR ;
Raven, N ;
Robinson, C ;
Robson, R ;
Sharp, RJ .
EXTREMOPHILES, 2000, 4 (05) :305-313
[3]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[4]   BACILLUS-ACIDOCALDARIUS SP.NOV., AN ACIDOPHILIC THERMOPHILIC SPORE-FORMING BACTERIUM [J].
DARLAND, G ;
BROCK, T .
JOURNAL OF GENERAL MICROBIOLOGY, 1971, 67 (JUL) :9-&
[5]   BACILLUS-ACIDOTERRESTRIS SP-NOV, A NEW THERMOTOLERANT ACIDOPHILE ISOLATED FROM DIFFERENT SOILS [J].
DEINHARD, G ;
BLANZ, P ;
PORALLA, K ;
ALTAN, E .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1987, 10 (01) :47-53
[6]   BACILLUS-CYCLOHEPTANICUS SP-NOV, A NEW THERMOACIDOPHILE CONTAINING OMEGA-CYCLOHEPTANE FATTY-ACIDS [J].
DEINHARD, G ;
SAAR, J ;
KRISCHKE, W ;
PORALLA, K .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1987, 10 (01) :68-73
[7]   FLUOROMETRIC DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEIC ACID HYBRIDIZATION IN MICRODILUTION WELLS AS AN ALTERNATIVE TO MEMBRANE-FILTER HYBRIDIZATION IN WHICH RADIOISOTOPES ARE USED TO DETERMINE GENETIC RELATEDNESS AMONG BACTERIAL STRAINS [J].
EZAKI, T ;
HASHIMOTO, Y ;
YABUUCHI, E .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03) :224-229
[8]   Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus [J].
Goto, K ;
Omura, T ;
Hara, Y ;
Sadaie, Y .
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2000, 46 (01) :1-8
[9]  
GOTO K, 2002, IN PRESS J GEN APPL
[10]  
Goto K., 2000, J ANTIBACT ANTIFUNG, V28, p499e508