Tyrosinated, but not detyrosinated, α-tubulin is present in root tip cells

被引：11

作者：

Gilmer, S

Clay, P

MacRae, TH

Fowke, LC

机构：

[1] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada

[2] Dalhousie Univ, Dept Biol, Halifax, NS, Canada

来源：

PROTOPLASMA | 1999年 / 210卷 / 1-2期

关键词：

posttranslational modification; tubulin; tyrosination; conifer; angiosperm;

D O I：

10.1007/BF01314959

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The distribution of tyrosinated and detyrosinated tubulin in microtubule arrays of pine and onion cells was investigated by immunofluorescence techniques. Staining of isolated cells and methacrylate sections of Pinus radiata and Allium cepa root tips indicated that all microtubule structures contained tyrosinated tubulin but not the posttranslationally modified detyrosinated tubulin. The detyrosinated tubulin epitope was however, created in vitro by treating both sections and fixed whole cells with carboxypeptidase A.

引用

页码：92 / 98

页数：7

共 28 条

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