Occurrence of free N-glycans in pea (Pisum sativum L) seedlings

被引：36

作者：

Kimura, Y ^{[1
]}

Takagi, S ^{[1
]}

Shiraishi, T ^{[1
]}

机构：

[1] OKAYAMA UNIV, FAC AGR, DEPT BIOL FUNCT & GENET RESOURCES SCI, OKAYAMA 700, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 05期

关键词：

free N-glycans; pea seedling; endo-beta-N-acetylglucosaminidase; peptide; N-glycanase; ion-spray mass spectrometry;

D O I：

10.1271/bbb.61.924

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Free N-glycans have been found in pea seedlings, These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con A-Sepharose affinity chromatography, and size fractionation HPLC, These structures of pyridylaminated free N-glycans were analyzed by exomannosidase digestions and ion-spray tandem mass spectrometry. The structural analyses showed that the several oligomannose-type sugar chains having one GlcNAc residue at the reducing-end side occur in the seedlings, suggesting the endo-beta-N-acetylglucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228-232 (1996)] should be involved in the release of oligomannose-type N-glycans from the storage glycoproteins [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 1841-1850 (1996)] during the germination of pea seeds.

引用

页码：924 / 926

页数：3

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