TRPV3 and TRPV4 mediate warmth-evoked currents in primary mouse keratinocytes

被引:212
作者
Chung, MK
Lee, H
Mizuno, A
Suzuki, M
Caterina, MJ
机构
[1] Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA
[3] Jichi Med Sch, Dept Pharmacol, Minami Kawachi, Tochigi 3290498, Japan
关键词
D O I
10.1074/jbc.M401872200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recently, a family of temperature-activated ion channels has been identified in mammalian and nonmammalian species that appear to contribute to thermosensation. Two of these proteins, TRPV3 and TRPV4, are ion channels activated by modest increases in ambient temperature. Localization studies have indicated that both proteins, in addition to being expressed in sensory neurons, are also expressed in skin keratinocytes. These and other findings have suggested that keratinocytes might act in concert with sensory neurons to perceive our thermal environment. In this study, we demonstrate that primary keratinocytes isolated from mouse skin exhibit two distinct heat-evoked current responses to mild increases in ambient temperature. The more common of these response types bears considerable similarity to responses mediated by recombinant TRPV4, is absent in mice lacking this ion channel, and is restored upon TRPV4 reintroduction. The second, rarer response strongly resembles those mediated by recombinant TRPV3. Together, these findings demonstrate that keratinocytes can indeed act as thermosensory cells and that they do so via at least two distinct transduction mechanisms.
引用
收藏
页码:21569 / 21575
页数:7
相关论文
共 40 条
  • [1] Hypotonicity induces TRPV4-mediated nociception in rat
    Alessandri-Haber, N
    Yeh, JJ
    Boyd, AE
    Parada, CA
    Chen, XJ
    Reichling, DB
    Levine, JD
    [J]. NEURON, 2003, 39 (03) : 497 - 511
  • [2] The vanilloid receptor: A molecular gateway to the pain pathway
    Caterina, MJ
    Julius, D
    [J]. ANNUAL REVIEW OF NEUROSCIENCE, 2001, 24 : 487 - 517
  • [3] The capsaicin receptor: a heat-activated ion channel in the pain pathway
    Caterina, MJ
    Schumacher, MA
    Tominaga, M
    Rosen, TA
    Levine, JD
    Julius, D
    [J]. NATURE, 1997, 389 (6653) : 816 - 824
  • [4] A capsaicin-receptor homologue with a high threshold for noxious heat
    Caterina, MJ
    Rosen, TA
    Tominaga, M
    Brake, AJ
    Julius, D
    [J]. NATURE, 1999, 398 (6726) : 436 - 441
  • [5] Warm temperatures activate TRPV4 in mouse 308 keratinocytes
    Chung, MK
    Lee, H
    Caterina, MJ
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (34) : 32037 - 32046
  • [6] Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2
    Delany, NS
    Hurle, M
    Facer, P
    Alnadaf, T
    Plumpton, C
    Kinghorn, I
    See, CG
    Costigan, M
    Anand, P
    Woolf, CJ
    Crowther, D
    Sanseau, P
    Tate, SN
    [J]. PHYSIOLOGICAL GENOMICS, 2001, 4 (03) : 165 - 174
  • [7] P2X purinergic receptor antagonist accelerates skin barrier repair and prevents epidermal hyperplasia induced by skin barrier disruption
    Denda, M
    Inoue, K
    Fuziwara, S
    Denda, S
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2002, 119 (05) : 1034 - 1040
  • [8] Immunoreactivity of VR1 on epidermal keratinocyte of human skin
    Denda, M
    Fuziwara, S
    Inoue, K
    Denda, S
    Akamatsu, H
    Tomitaka, A
    Matsunaga, K
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 285 (05) : 1250 - 1252
  • [9] Temperature-modulated diversity of TRPV4 channel gating - Activation by physical stresses and phorbol ester derivatives through protein kinase C-dependent and -independent pathways
    Gao, X
    Wu, L
    O'Neil, RG
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (29) : 27129 - 27137
  • [10] Güler AD, 2002, J NEUROSCI, V22, P6408