Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy

被引：21

作者：

Liu, L. ^{[1
]}

Qu, J. ^{[1
]}

Lin, Z. ^{[1
]}

Wang, L. ^{[1
]}

Fu, Z. ^{[1
]}

Guo, B. ^{[1
]}

Niu, H. ^{[1
]}

机构：

[1] Inst Optoelect, Minist Educ, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Guangdong, Peoples R China

来源：

APPLIED PHYSICS B-LASERS AND OPTICS | 2006年 / 84卷 / 03期

关键词：

D O I：

10.1007/s00340-006-2314-y

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split the near-infrared light of a mode-locked titanium:sapphire femtosecond laser into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3x3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system of the instrument, we demonstrate the acquisition of a five-dimensional data set combining lifetime and spectral resolutions in biological fluorescence imaging.

引用

页码：379 / 383

页数：5

共 18 条

[1] Multi-wavelength TCSPC lifetime imaging [J].