A new approach to immunological sexing of sperm

被引：43

作者：

Blecher, SR ^{[1
]}

Howie, R ^{[1
]}

Li, S ^{[1
]}

Detmar, J ^{[1
]}

Blahut, LM ^{[1
]}

机构：

[1] Univ Guelph, Dept Mol Biol & Genet, Guelph, ON N1G 2W1, Canada

来源：

THERIOGENOLOGY | 1999年 / 52卷 / 08期

关键词：

sperm sexing; immunology; post-meiotic transcription; sex-chromosome-specific proteins;

D O I：

10.1016/S0093-691X(99)00219-8

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A non-invasive, immunological method for sexing mammalian sperm would be of benefit to agricultural industries. This paper presents a new approach, based on the hypothesis that sex-specific proteins (SSPs) are evolutionarily more highly conserved than non-SSPs. Antibodies to non-SSPs were raised and used in an affinity procedure to remove non-SSPs and enrich for SSPs. Thereafter, using column chromatography, purified SSPs were obtained. Sex-specific antibodies (SSAbs) raised against these SSPs appear to bind to sex-chromosome-specific proteins (SCSPs) on the sperm membrane and make possible a sperm-sexing procedure. Antibodies to SCSPs were raised and used to identify putative SCSPs by affinity chromatography. The preliminary results presented here suggest that a viable immunological sperm sexing procedure can be developed. (C) 1999 by Elsevier Science Inc.

引用

页码：1309 / 1321

页数：13

共 36 条

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