Construction of a 1.2-Mb BAC/PAC contig of the porcine gene RYR1 region on SSC 6q1.2 and comparative analysis with HSA 19q13.13

被引：13

作者：

Martins-Wess, F

Voss-Nemitz, R

Drögemüller, C

Brenig, B

Leeb, T

机构：

[1] Hannover Sch Vet Med, Inst Anim Breeding & Genet, D-30559 Hannover, Germany

[2] Univ Gottingen, Inst Vet Med, D-37073 Gottingen, Germany

来源：

GENOMICS | 2002年 / 80卷 / 04期

关键词：

SSC; 6; HSA; 19; MMU; 7; RYR1; contig; comparative mapping; pig; malignant hyperthermia; stress susceptibility; QTL;

D O I：

10.1006/geno.2002.6846

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We screened a porcine bacterial artificial chromosome (BAC) and a P1 derived artificial chromosome (PAC) library to construct a sequence-ready similar to1.2-Mb BAC/PAC contig of the ryanodine receptor-1 gene (RYR1) region on porcine chromosome (SSC) 6q1.2. This genomic segment is of special interest because it harbors the locus for stress susceptibility in pigs and a putative quantitative trait locus for muscle growth. Detailed physical mapping of this gene-rich region allowed us to assign to this contig 17 porcine genes orthologous to known human chromosome 19 genes. Apart from the relatively well-characterized porcine gene RYR1, the other 16 genes represent novel chromosomal assignments and 14 genes have been cloned for the first time in pig. Comparative analysis of the porcine BAC/PAC contig with the human chromosome (HSA) 19q13.13 map revealed a completely conserved gene order of this segment between pig and human. A detailed porcine-human-mouse comparative map of this region was constructed.

引用

页码：416 / 422

页数：7

共 18 条

[1] Construction and characterization of a porcine P1-derived artificial chromosome (PAC) library covering 3.2 genome equivalents and cytogenetical assignment of six type I and type II loci [J].