High-performance liquid chromatography of methamphetamine and its related compounds in human urine following derivatization with fluorescein isothiocyanate

被引:26
作者
AlDirbashi, O [1 ]
Kuroda, N [1 ]
Akiyama, S [1 ]
Nakashima, K [1 ]
机构
[1] NAGASAKI UNIV,SCH PHARMACEUT SCI,NAGASAKI 852,JAPAN
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 695卷 / 02期
关键词
methamphetamine; amphetamine; norephedrine; p-hydroxymethamphetamine; fluorescein isothiocyanate;
D O I
10.1016/S0378-4347(97)00168-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A high-performance liquid chromatographic method with fluorescence detection for the determination of methamphetamine and its related compounds is reported. Methamphetamine, amphetamine, norephedrine, p-hydroxymethamphetamine and 1-phenylethylamine as an internal standard were extracted from human urine, derivatized with fluorescein-4-isothiocyanate, and then separated on a reversed-phase column within 36 min. The fluorescence intensity of the effluent was monitored at excitation and emission wavelengths of 496 and 518 nm, respectively. Calibration curves were confirmed to be linear up to at least 100 pmol on the column with a correlation coefficient (r) of 0.994-0.999 for the target compounds. The detection limits (S/N = 3) were 55-105 fmol per 20-mu l injection. The method was successfully applied to urine samples taken from methamphetamine addicts. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:251 / 258
页数:8
相关论文
共 21 条
  • [1] BUZAS A, 1950, B SOC CHIM FR, P139
  • [2] METABOLISM OF [C-14]METHAMPHETAMINE IN MAN, GUINEA-PIG AND RAT
    CALDWELL, J
    DRING, LG
    WILLIAMS, RT
    [J]. BIOCHEMICAL JOURNAL, 1972, 129 (01) : 11 - &
  • [3] INTERPRETATION OF METHAMPHETAMINE AND AMPHETAMINE ENANTIOMER DATA
    CODY, JT
    SCHWARZHOFF, R
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1993, 17 (06) : 321 - 326
  • [4] DESIGN AND DEVELOPMENT OF A SINGLE-REAGENT POLARIZATION FLUOROIMMUNOASSAY FOR METHAMPHETAMINE
    EREMIN, SA
    SCHIAVETTA, DE
    LOTEY, H
    SMITH, DS
    LANDON, J
    [J]. THERAPEUTIC DRUG MONITORING, 1988, 10 (03) : 327 - 332
  • [5] EREMIN SA, 1987, CLIN CHEM, V33, P1903
  • [6] GAN BK, 1991, J FORENSIC SCI, V36, P1331
  • [7] DETERMINATION OF AMPHETAMINE AND METHAMPHETAMINE IN BLOOD BY DERIVATIZATION WITH PERFLUOROOCTANOYL CHLORIDE AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
    GJERDE, H
    HASVOLD, I
    PETTERSEN, G
    CHRISTOPHERSEN, AS
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1993, 17 (02) : 65 - 68
  • [8] HAYAKAWA K, 1993, BIOL PHARM BULL, V16, P817
  • [9] DETERMINATION OF METHAMPHETAMINE, AMPHETAMINE AND PIPERIDINE IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CHEMILUMINESCENCE DETECTION
    HAYAKAWA, K
    IMAIZUMI, N
    ISHIKURA, H
    MINOGAWA, E
    TAKAYAMA, N
    KOBAYASHI, H
    MIYAZAKI, M
    [J]. JOURNAL OF CHROMATOGRAPHY, 1990, 515 : 459 - 466
  • [10] HAYAKAWA K, 1989, J CHROMATOGR, V464, P343