Effects of Low-Dose Recombinant Interleukin 2 to Promote T-Regulatory Cells in Alopecia Areata

被引:195
作者
Castela, Emeline [1 ]
Le Duff, Florence [1 ,2 ]
Butori, Catherine [3 ]
Ticchioni, Michel [4 ]
Hofman, Paul [3 ]
Bahadoran, Philippe [1 ,2 ]
Lacour, Jean-Philippe [1 ]
Passeron, Thierry [1 ,5 ]
机构
[1] Univ Hosp Nice, Dept Dermatol, Nice, France
[2] Univ Hosp Nice, CRC, Nice, France
[3] Univ Hosp Nice, Lab Clin & Expt Pathol, Nice, France
[4] Univ Hosp Nice, Immunol Lab, Nice, France
[5] Mediterranean Ctr Mol Med, Team 12, U1065, INSERM, Nice, France
关键词
IN-VIVO; DISEASE; FOXP3;
D O I
10.1001/jamadermatol.2014.504
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100227 [皮肤病学];
摘要
IMPORTANCE An impaired inhibitory function of circulating CD4(+)CD25(+) regulatory T (Treg) cells was reported to play a key role in alopecia areata (AA). We report the first use to our knowledge of low-dose interleukin 2 for treating severe AA by promoting the recruitment of Treg cells. OBSERVATIONS We conducted a prospective open pilot study in 5 patients with severe AA resistant to previous systemic treatments. Subcutaneous interleukin 2 (1.5 million IU/d) was administered during 5 days, followed by three 5-day courses of 3 million IU/d at weeks 3, 6, and 9. The primary outcome was the evolution of the Severity of Alopecia Tool (SALT) score, evaluated by 2 independent investigators on standardized photographs. Lesional skin biopsy specimens and peripheral blood lymphocyte phenotype were analyzed. The median SALT score went from 82 (range, 63-100) at baseline to 69 (range, 28-100) at 6 months. Immunochemical analysis revealed the appearance or a notable increase in Treg cell count in 4 of 5 patients at the end of the treatment compared with baseline. No serious adverse event was reported. CONCLUSIONS AND RELEVANCE The partial regrowth achieved in 4 of 5 patients and the recruitment of Treg cells in lesional skin support the interest of promoting Treg cells for treating AA. Further investigations are now required to confirm and to optimize the design in order to enhance the Treg cell response.
引用
收藏
页码:748 / 751
页数:4
相关论文
共 15 条
[1]
Psychiatric comorbidities in patients with alopecia areata in Taiwan: a case-control study [J].
Chu, S-Y. ;
Chen, Y-J. ;
Tseng, W-C. ;
Lin, M-W. ;
Chen, T-J. ;
Hwang, C-Y. ;
Chen, C-C. ;
Lee, D-D. ;
Chang, Y-T. ;
Wang, W-J. ;
Liu, H-N. .
BRITISH JOURNAL OF DERMATOLOGY, 2012, 166 (03) :525-531
[2]
Interventions for alopecia areata [J].
Delamere, F. M. ;
Sladden, M. M. ;
Dobbins, H. M. ;
Leonardi-Bee, J. .
COCHRANE DATABASE OF SYSTEMATIC REVIEWS, 2008, (02)
[3]
Autoimmune hair loss (alopecia areata) transferred by T lymphocytes to human scalp explants on SCID mice [J].
Gilhar, A ;
Ullmann, Y ;
Berkutzki, T ;
Assy, B ;
Kalish, RS .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (01) :62-67
[4]
Medical Progress Alopecia Areata [J].
Gilhar, Amos ;
Etzioni, Amos ;
Paus, Ralf .
NEW ENGLAND JOURNAL OF MEDICINE, 2012, 366 (16) :1515-1525
[5]
Interleukin-2 and Regulatory T Cells in Graft-versus-Host Disease [J].
Koreth, John ;
Matsuoka, Ken-ichi ;
Kim, Haesook T. ;
McDonough, Sean M. ;
Bindra, Bhavjot ;
Alyea, Edwin P., III ;
Armand, Philippe ;
Cutler, Corey ;
Ho, Vincent T. ;
Treister, Nathaniel S. ;
Bienfang, Don C. ;
Prasad, Sashank ;
Tzachanis, Dmitrios ;
Joyce, Robin M. ;
Avigan, David E. ;
Antin, Joseph H. ;
Ritz, Jerome ;
Soiffer, Robert J. .
NEW ENGLAND JOURNAL OF MEDICINE, 2011, 365 (22) :2055-2066
[6]
Transfer of CD8+ cells induces localized hair loss whereas CD4+/CD25- cells promote systemic alopecia areata and CD4+/CD25+ cells blockade disease onset in the C3H/HeJ mouse model [J].
McElwee, KJ ;
Freyschmidt-Paul, P ;
Hoffmann, R ;
Kissling, S ;
Hummel, S ;
Vitacolonna, M ;
Zöller, M .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2005, 124 (05) :947-957
[7]
Alopecia areata investigational assessment guidelines - Part II [J].
Olsen, EA ;
Hordinsky, MK ;
Price, VH ;
Roberts, JL ;
Shapiro, J ;
Canfield, D ;
Duvic, M ;
King, LE ;
McMichael, AJ ;
Randall, VA ;
Turner, ML ;
Sperling, L ;
Whiting, DA ;
Norris, D .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 2004, 51 (03) :440-447
[8]
Association of Two Clones Allows for Optimal Detection of Human FOXP3 [J].
Presicce, Pietro ;
Moreno-Fernandez, Maria E. ;
Lages, Celine S. ;
Orsborn, Kris I. ;
Chougnet, Claire A. .
CYTOMETRY PART A, 2010, 77A (06) :571-579
[9]
Efficacy of topical sensitizers in the treatment of alopecia areata [J].
Rokhsar, CK ;
Shupack, JL ;
Vafai, JJ ;
Washenik, K .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1998, 39 (05) :751-761
[10]
IL-2-IgG2b fusion protein suppresses murine contact hypersensitivity in vivo [J].
Rückert, R ;
Brandt, K ;
Hofmann, U ;
Bulfone-Paus, S ;
Paus, R .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2002, 119 (02) :370-376