Polymorphic restriction patterns of ribosomal internal transcribed spacers in the biocontrol fungus Puccinia carduorum correlate with weed host origin

被引：11

作者：

Berthier, YT ^{[1
]}

Bruckart, WL ^{[1
]}

Chaboudez, P ^{[1
]}

Luster, DG ^{[1
]}

机构：

[1] ARS,USDA,FT DETRICK,FREDERICK,MD 21702

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 08期

关键词：

D O I：

10.1128/AEM.62.8.3037-3041.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The internal transcribed spacer (ITS) regions of ribosomal DNA were amplified by PCR and used to develop genetic markers for isolates of Puccinia carduorum being evaluated for biological control of Carduus thoermeri (musk thistle). Unique patterns were produced upon restriction of ITS DNA amplified from four separate Puccinia spp. Restriction patterns of ITS DNA of isolates of P. carduorum from Carduus acanthoides and C. thoermeri were distinct from those of P. carduorum from Carduus tenuiflorus and Carduus pycnocephalus. By this technique, isolates of P. carduorum from four different weed hosts can be differentiated from other Puccinia spp. and separated into two host groups.

引用

页码：3037 / 3041

页数：5

共 25 条

[11] HETEROBASIDION-ANNOSUM 5.8S RIBOSOMAL DNA AND INTERNAL TRANSCRIBED SPACER SEQUENCE - RAPID IDENTIFICATION OF EUROPEAN INTERSTERILITY GROUPS BY RIBOSOMAL DNA RESTRICTION POLYMORPHISM [J].