Conserved ribonuclease, Eri1, negatively regulates heterochromatin assembly in fission yeast

被引:48
作者
Iida, Tetsushi [1 ]
Kawaguchi, Rika [1 ]
Nakayama, Jun-ichi [1 ]
机构
[1] RIKEN, Ctr Dev Biol, Lab Chromatin Dynam, Kobe, Hyogo 6500047, Japan
关键词
RNA INTERFERENCE; LYSINE-9; METHYLATION; EPIGENETIC CONTROL; C-ELEGANS; RITS; ESTABLISHMENT; INTERACT; PATHWAYS;
D O I
10.1016/j.cub.2006.05.061
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
RNA interference (RNA!) is a conserved silencing mechanism that has widespread roles in RNA degradation, translational repression, and the epigenetic control of chromatin structure [1]. In fission yeast, heterochromatin assembly requires RNAi machinery and is initiated by small interference RNAs (siRNAs) derived from heterochromatic regions and by the RNA-induced transcriptional silencing (RITS) complex [27]. Although recent studies have been successful in uncovering the functions of effector complexes in the RNAi pathway [4, 5, 8-10], exactly how heterochromatic siRNAs are processed and function in assembling heterochromatin remains unclear. In this study we focused on a conserved ribonuclease, Eri1, which was originally identified as a negative regulator of RNAi in C. elegans [11], and show the importance of the Eri1 protein in RNAi-mediated heterochromatin assembly in fission yeast. Eri1 specifically degrades double-stranded s1RNAs through two functional domains and represses the accumulation of cellular siRNAs in vivo. Deletion of eri1+ causes an increase in siRNAs associated with the RITS complex and enhances heterochromatic silencing, which is accompanied by increased levels of histone H3-K9 methylation and the Swi6 protein. Our findings suggest that the fission yeast Eri1 controls the accumulation of heterochromatic siRNAs and negatively regulates the RNAi-mediated heterochromatin assembly.
引用
收藏
页码:1459 / 1464
页数:6
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