New class of poly(vinyl alcohol) polymers as column-chromatography stationary phases for Candida rugosa lipase isoforms separation

被引：10

作者：

Battinelli, L

Cernia, E

Delbo, M

Ortaggi, G

Pala, A

Soro, S

机构：

[1] UNIV ROMA LA SAPIENZA,DIPARTIMENTO CHIM,I-00185 ROME,ITALY

[2] UNIV ROMA LA SAPIENZA,LAB BIOCHIM ORMONI SESSUALI,FAC MED,CLIN OSTETR & GINECOL 2,I-00185 ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 753卷 / 01期

关键词：

Candida rugosa; stationary phases; LC; polyvinyl alcohol fatty acids; enzymes; lipases;

D O I：

10.1016/S0021-9673(96)00482-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of new stationary phases of cross-linked polyvinyl alcohol esterified with various linear fatty acids is described. The physico-chemical properties of these polymers are reported, including electron microscopy and swelling measurements. Batch adsorption experiments were performed in order to characterize the basic separative properties of these phases. Cross-linked polyvinyl alcohol esterified with dodecanoic acid was used for hydrophobic interaction chromatography of a commercial crude preparation of Candida rugosa lipase. Characterization of the purified fractions was carded out via native electrophoresis and sodium dodecyl sulfate-polyacrylamide electrophoresis.

引用

页码：47 / 55

页数：9

共 12 条

[1]

ALBERGHINA L, 1991, GBF MONOGRAPHY, V16, P369

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] THE ESTERASE PROFILE OF A LIPASE FROM CANDIDA-CYLINDRACEA [J].