Combined effect of pH and temperature during electroreduction of whey proteins

被引:22
作者
Bazinet, L
Lamarche, F
Boulet, M
Amiot, J
机构
[1] UNIV LAVAL,CTR RECH SCI & TECHNOL LAIT,LAVAL,PQ G1K 7P4,CANADA
[2] AGR CANADA,CTR FOOD RES & DEV,ST HYACINTHE,PQ J2S 8E3,CANADA
关键词
membrane electrolysis; whey; protein; electroreduction; aggregation;
D O I
10.1021/jf9602434
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Electroreduction was conducted at various pH values (4, 6, and 8) and temperatures (25, 45, and 65 degrees C) to evaluate the effect of these parameters on the structure of whey proteins. A preliminary study with cystine as a disulfide bond (S-S) reduction model indicated that the use of a reticulated vitreous carbon cathode and an anode/cathode voltage difference of 6 V was suitable for the electroreduction of whey proteins. Up to a 39% reduction of whey protein S-S bonds was obtained by electroreduction for 3 h at pH 6.0 and 65 degrees C, with pH and temperature accounting for 33% and electroreduction for 67% of the result. New molecules were formed by cr-lactalbumin aggregation or co-aggregation with B-lactoglobulin or fragments of bovine serum albumin The contribution of pH and temperature would be to produce free SH in addition to the ones released by electroreduction, and to increase thiol-disulfide interchange reactions.
引用
收藏
页码:101 / 107
页数:7
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