PS-100 and NF 70-200 double immunolabeling for human digital skin Meissner corpuscle 3D imaging

被引:18
作者
Guinard, D
Usson, Y
Guillermet, C
Saxod, R
机构
[1] Univ Grenoble 1, LAPSEN U Inserm 318, Equipe Neurobiol Dev, F-38043 Grenoble 9, France
[2] Univ Grenoble 1, Inst Albert Bonniot, Ctr Hosp, Pathol Cellulaire Lab, F-38041 Grenoble, France
关键词
Meissner corpuscles; immunolabeling; confocal microscopy; mechanoreceptors; 3D reconstruction; cutaneous innervation; finger;
D O I
10.1177/002215540004800215
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
For detailed study of complex structures such as corpuscular mechanoreceptors, confocal microscopy can be used with multiple immunolabeling that identifies specifically different subcomponents. In addition, anatomic interpretation is enhanced by three-dimensional reconstruction. Confocal laser micrographs, reconstructed from serial images 1 mu m thick of human skin Meissner corpuscles simultaneously immunostained for neurofilaments (NF 70-200) and protein S-100 (PS-100), clearly reveal the complex 3D relationship between Schwann-related lamellar cells immunoreactive for PS-100 and the nerve fibers marked by NF 70-200. The nerve fiber, after branching into the corpuscle, divides into several ramifications, presenting discoidal expansions and flattened fringed sections. The mean nerve diameter was 4 mu m +/- 1 (2-5 mu m) and the mean size of the discoidal expansions was 15 mu m +/- 1 (7-30 mu m). Corpuscle size varied from 30-140 +/- 1 mu m in length and from 20-60 +/- 1 mu m in diameter. This study confirms the presence of neural discoidal areas in Meissner's corpuscles, which are probably involved to some extent with the transduction process. Despite the accuracy of immunolabeling and imaging, an extracorpuscular neural network was never observed in the vicinity of corpuscles, thus giving doubt as to their existence.
引用
收藏
页码:295 / 302
页数:8
相关论文
共 46 条
[21]  
HARO JJ, 1991, EUR J MORPHOL, V29, P271
[22]  
HASCHIMOTO K, 1973, J INVEST DERMATOL, V60, P20
[23]   FINE-STRUCTURE OF DIGITAL CORPUSCLE OF MOUSE TOE PAD, WITH SPECIAL REFERENCE TO NERVE-FIBERS [J].
IDE, C .
AMERICAN JOURNAL OF ANATOMY, 1976, 147 (03) :329-355
[24]  
Iggo Ainsley, 1974, The Peripheral Nervous System, P347, DOI 10.1007/978-1-4615-8699-9_14
[25]   MEISSNERS AND PACINIAN CORPUSCLES AS STUDIES BY IMMUNOHISTOCHEMISTRY FOR S-100 PROTEIN, NEURON-SPECIFIC ENOLASE AND NEUROFILAMENT PROTEIN [J].
IWANAGA, T ;
FUJITA, T ;
TAKAHASHI, Y ;
NAKAJIMA, T .
NEUROSCIENCE LETTERS, 1982, 31 (02) :117-121
[26]   AN IMMUNOCYTOCHEMICAL STUDY OF CUTANEOUS INNERVATION AND THE DISTRIBUTION OF NEUROPEPTIDES AND PROTEIN GENE-PRODUCT 9.5 IN MAN AND COMMONLY EMPLOYED LABORATORY-ANIMALS [J].
KARANTH, SS ;
SPRINGALL, DR ;
KUHN, DM ;
LEVENE, MM ;
POLAK, JM .
AMERICAN JOURNAL OF ANATOMY, 1991, 191 (04) :369-383
[27]   THE INNERVATION OF HUMAN EPIDERMIS [J].
KENNEDY, WR ;
WENDELSCHAFERCRABB, G .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 1993, 115 (02) :184-190
[28]  
MATSUOKA S, 1983, Journal of Dermatology (Tokyo), V10, P205
[29]   A REVIEW OF RECENT ADVANCES IN STEREOLOGY FOR QUANTIFYING NEURAL STRUCTURE [J].
MAYHEW, TM .
JOURNAL OF NEUROCYTOLOGY, 1992, 21 (05) :313-328
[30]   THE STRUCTURE AND FUNCTION OF CUTANEOUS SENSORY RECEPTORS [J].
MUNGER, BL ;
IDE, C .
ARCHIVES OF HISTOLOGY AND CYTOLOGY, 1988, 51 (01) :1-34