Production of chimeric monoclonal antibodies by genetically manipulated chickens

被引：36

作者：

Kamihira, Masamichi ^{[1
]}

Kawabe, Yoshinori ^{[1
]}

Shindo, Takuya ^{[2
]}

Ono, Ken-ichiro ^{[3
]}

Esaka, Kazuhisa ^{[3
]}

Yamashita, Takashi ^{[2
]}

Nishijima, Ken-ichi ^{[3
]}

Iijima, Shinji ^{[3
]}

机构：

[1] Kyushu Univ, Dept Chem Engn, Fac Engn, Nishi Ku, Fukuoka 8190395, Japan

[2] Kaneka Corp, Takasago, Hyogo 6768688, Japan

[3] Nagoya Univ, Grad Sch Engn, Dept Biotechnol, Chikusa Ku, Nagoya, Aichi 4648603, Japan

来源：

JOURNAL OF BIOTECHNOLOGY | 2009年 / 141卷 / 1-2期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Transgenic avian bioreactor; Chimeric monoclonal antibody; Retroviral vector; N-linked oligosaccharide chain structure; RECOMBINANT ANIMAL-CELLS; FC-FUSION PROTEINS; TRANSGENIC CHICKENS; EGG-WHITE; RETROVIRAL VECTOR; IMMUNOGLOBULIN-G; EXPRESSION; OLIGOSACCHARIDES; THERAPEUTICS; HENS;

D O I：

10.1016/j.jbiotec.2009.02.022

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Genetically manipulated chickens producing chimeric monoclonal antibodies were generated by injecting retroviral vectors encoding genes for the heavy and light chains of antibodies into developing embryos. The transgene was detected in all chickens that hatched, and they stably produced the chimeric antibodies in their serum. After sexual maturation, the antibodies were also produced in eggs laid by the manipulated hens. The stable antibody production was observed both in egg white and yolk throughout the breeding period. The chimeric antibodies produced by the chickens were properly assembled and exhibited antigen-binding activities. Furthermore, we characterized the structures of the Winked oligosaccharide chains added to the Fc-region of the recombinant antibodies produced in the serum, egg white and yolk of the chickens. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：18 / 25

页数：8

共 44 条

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