Apoptosis induction by γ-tocotrienol in human hepatoma Hep3B cells

被引：82

作者：

Sakai, Midori ^{[1
]}

Okabe, Masaaki ^{[1
]}

Tachibana, Hirofumi ^{[1
]}

Yamada, Koji ^{[1
]}

机构：

[1] Kyushu Univ, Fac Agr, Higashi Ku, Fukuoka 8128581, Japan

来源：

JOURNAL OF NUTRITIONAL BIOCHEMISTRY | 2006年 / 17卷 / 10期

关键词：

tocotrienol; human hepatoma Hep3B cells; apoptosis;

D O I：

10.1016/j.jnutbio.2005.11.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We evaluated the antitumor activity of tocotrienol (T3) on human hepatoma Hep3B cells. At first, we examined the effect of T3 on the proliferation of human hepatoma Hep3B cells and found that gamma-T3 inhibited cell proliferation at lower concentrations and shorter treatment times than alpha-T3. Then, we examined the effect of gamma-T3 apoptosis induction and found that gamma-T3 induced poly (ADP-ribose) polymerase (PARP) cleavage and stimulated a rise in caspase-3 activity. In addition, gamma-T3 stimulated a rise in caspase-8 and caspase-9 activities. We also found that gamma-T3-induced apoptotic cell death was accompanied by up-regulation of Bax and a rise in the fragments of Bid and caspase-8. These data indicate that gamma-T3 induced apoptosis in Hep3B cells and that caspase-8 and caspase-9 were involved in apoptosis induction. Moreover, these results suggest that Bax and Bid regulated apoptosis induction by gamma-T3. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：672 / 676

页数：5

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