Label-Free Electrical Detection of Cardiac Biomarker with Complementary Metal-Oxide Semiconductor-Compatible Silicon Nanowire Sensor Arrays

被引：222

作者：

Chua, Jay Huiyi ^{[1
]}

Chee, Ru-Ern ^{[1
]}

Agarwal, Ajay ^{[1
]}

Wong, She Mein ^{[1
]}

Zhang, Guo-Jun ^{[1
]}

机构：

[1] Agcy Sci Technol & Res, Inst Microelect, Singapore 117685, Singapore

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 15期

关键词：

TROPONIN-T; DNA HYBRIDIZATION; BIOSENSORS; NANOSENSORS; SPECIFICITY; MOLECULES; INJURY;

D O I：

10.1021/ac901157x

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Arrays of highly ordered silicon nanowire (SiNW) clusters are fabricated using complementary metal-oxide semiconductor (CMOS) field effect transistor-compatible technology, and the ultrasensitive, label-free, electrical detection of cardiac biomarker in real time using the array sensor is presented. The successful detection of human cardiac troponin-T (cTnT) has been demonstrated in an assay buffer solution of concentration down to 1 fg/mL, as well as in an undiluted human serum environment of concentration as low as 30 fg/mL. The high specificity, selectivity, and swift response time of the SiNWs to the presence of ultralow concentrations of a target protein in a biological analyte solution, even in the presence of a high total protein concentration, paves the way for the development of a medical diagnostic system for point-of-care application that is able to provide an early and accurate indication of cardiac cellular necrosis.

引用

页码：6266 / 6271

页数：6

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