Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp israelensis

被引:241
作者
Berry, C
O'Neil, S
Ben-Dov, E
Jones, AF
Murphy, L
Quail, MA
Holden, MTG
Harris, D
Zaritsky, A
Parkhill, J
机构
[1] Cardiff Univ, Cardiff Sch Biosci, Cardiff CF10 3US, S Glam, Wales
[2] Sanger Inst, Hinxton Cambridge CB10 1SA, England
[3] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel
关键词
D O I
10.1128/AEM.68.10.5082-5095.2002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The entire 127,923-bp sequence of the toxin-encoding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis is presented and analyzed. In addition to the four known Cry and two known Cyt toxins, a third Cyt-type sequence was found with an additional C-terminal domain previously unseen in such proteins. Many plasmid-encoded genes could be involved in several functions other than toxin production. The most striking of these are several genes potentially affecting host sporulation and germination and a set of genes for the production and export of a peptide antibiotic.
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页码:5082 / 5095
页数:14
相关论文
共 54 条
[1]   PLASMID DEOXYRIBONUCLEIC-ACID IN STRAINS OF BACILLUS-SPHAERICUS AND IN BACILLUS-MORITAI [J].
ABE, K ;
FAUST, RM ;
BULLA, LA .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1983, 41 (03) :328-335
[2]   PlcR is a pleiotropic regulator of extracellular virulence factor gene expression in Bacillus thuringiensis [J].
Agaisse, H ;
Gominet, M ;
Okstad, OA ;
Kolsto, AB ;
Lereclus, D .
MOLECULAR MICROBIOLOGY, 1999, 32 (05) :1043-1053
[3]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[4]   COMPLETE NUCLEOTIDE-SEQUENCE OF THE BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS PLASMID PTX14-3 AND ITS CORRELATION WITH BIOLOGICAL PROPERTIES [J].
ANDRUP, L ;
DAMGAARD, J ;
WASSERMANN, K ;
BOE, L ;
MADSEN, SM ;
HANSEN, FG .
PLASMID, 1994, 31 (01) :72-88
[5]   InterPro - an integrated documentation resource for protein families, domains and functional sites [J].
Apweiler, R ;
Attwood, TK ;
Bairoch, A ;
Bateman, A ;
Birney, E ;
Biswas, M ;
Bucher, P ;
Cerutti, L ;
Corpet, F ;
Croning, MDR ;
Durbin, R ;
Falquet, L ;
Fleischmann, W ;
Gouzy, J ;
Hermjakob, H ;
Hulo, N ;
Jonassen, I ;
Kahn, D ;
Kanapin, A ;
Karavidopoulou, Y ;
Lopez, R ;
Marx, B ;
Mulder, NJ ;
Oinn, TM ;
Pagni, M ;
Servant, F ;
Sigrist, CJA ;
Zdobnov, EM .
BIOINFORMATICS, 2000, 16 (12) :1145-1150
[6]   Refined, circular restriction map of the Bacillus thuringiensis subsp israelensis plasmid carrying the mosquito larvicidal genes [J].
Ben-Dov, E ;
Nissan, G ;
Pelleg, N ;
Manasherob, R ;
Boussiba, S ;
Zaritsky, A .
PLASMID, 1999, 42 (03) :186-191
[7]   Restriction map of the 125-kilobase plasmid of Bacillus thuringiensis subsp israelensis carrying the genes that encode delta-endotoxins active against mosquito larvae [J].
BenDov, E ;
Einav, M ;
Peleg, N ;
Boussiba, S ;
Zaritsky, A .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (09) :3140-3145
[8]   ISOLATION OF A BACILLUS-THURINGIENSIS RNA-POLYMERASE CAPABLE OF TRANSCRIBING CRYSTAL PROTEIN GENES [J].
BROWN, KL ;
WHITELEY, HR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (12) :4166-4170
[9]   Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins [J].
Crickmore, N ;
Zeigler, DR ;
Feitelson, J ;
Schnepf, E ;
Van Rie, J ;
Lereclus, D ;
Baum, J ;
Dean, DH .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (03) :807-+
[10]  
CRICKMORE N, 1995, FEMS MICROBIOL LETT, V131, P249