Expanded coverage of the human heart mitochondrial proteome using multidimensional liquid chromatography coupled with tandem mass spectrometry

被引：79

作者：

Gaucher, SP

Taylor, SW

Fahy, E

Zhang, B

Warnock, DE

Ghosh, SS

Gibson, BW

机构：

[1] MitoKor, San Diego, CA 92121 USA

[2] Buck Inst Age Res, Novato, CA 94945 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2004年 / 3卷 / 03期

关键词：

mitochondria; LC/MS/MS; MudPIT; MDLC;

D O I：

10.1021/pr034102a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent evidence suggests that mitochondria are closely linked with the aging process and degenerative disorders such as Alzheimer's disease and Parkinson's disease. Thus, there has been increasing interest in cataloging mitochondrial proteomes to identify potential diagnostic and therapeutic targets. We have previously reported results of a one-dimensional electrophoresis/liquid chromatography MS/MS study to characterize the proteome of normal human heart mitochondria (Taylor et al. Nat. Biotechnol. 2003, 21, 281-286). We now report two subsequent studies where multidimensional liquid chromatography MS/MS was investigated as an alternative means for characterizing the same sample.

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收藏

页码：495 / 505

页数：11

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