SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C-elegans embryos

被引：124

作者：

Bei, YX

Hogan, J

Berkowitz, LA

Soto, M

Rocheleau, CE

Pang, KM

Collins, J

Mello, CC ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Coconut Grove, FL 33133 USA

[2] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

[3] Univ New Hampshire, Dept Biochem & Mol Biol, Durham, NH 03824 USA

[4] Univ Tulsa, Dept Biol Sci, Tulsa, OK 74104 USA

来源：

DEVELOPMENTAL CELL | 2002年 / 3卷 / 01期

关键词：

D O I：

10.1016/S1534-5807(02)00185-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In early C. elegans embryos, signaling between a posterior blastomere, P2, and a ventral blastomere, EMS, specifies endoderm and orients the division axis of the EMS cell. Although Wnt signaling contributes to this polarizing interaction, no mutants identified to date abolish P2/EMS signaling. Here, we show that two tyrosine kinase-related genes, src-1 and mes-1, are required for the accumulation of phosphotyrosine between P2 and EMS. Moreover, src-1 and mes-1 mutants strongly enhance endoderm and EMS spindle rotation defects associated with Wnt pathway mutants. SRC-1 and MES-1 signal bidirectionally to control cell fate and division orientation in both EMS and P2. Our findings suggest that Wnt and Src signaling function in parallel to control developmental outcomes within a single responding cell.

引用

页码：113 / 125

页数：13

共 43 条

[21] Adherens junctions inhibit asymmetric division in the Drosophila epithelium
Lu, BW
Roegiers, F
Jan, LY
Jan, YN
[J]. NATURE, 2001, 409 (6819) : 522 - 525
[22] McEwen DG, 2000, DEVELOPMENT, V127, P3607
[23] MAP kinase and Wnt pathways converge to downregulate an HMG-domain repressor in Caenorhabditis elegans
Meneghini, MD
Ishitani, T
Carter, JC
Hisamoto, N
Ninomiya-Tsuji, J
Thorpe, CJ
Hamill, DR
Matsumoto, K
Bowerman, B
[J]. NATURE, 1999, 399 (6738) : 793 - 797
[24] Papkoff J, 1997, J BIOL CHEM, V272, P4536
[25] Cancer - Wnt signaling in oncogenesis and embryogenesis - a look outside the nucleus
Peifer, M
Polakis, P
[J]. SCIENCE, 2000, 287 (5458) : 1606 - 1609
[26] Drosophila Jun kinase regulates expression of decapentaplegic via the ETS-domain protein Aop and AP-1 transcription factor DJun during dorsal closure
RiesgoEscovar, JR
Hafen, E
[J]. GENES & DEVELOPMENT, 1997, 11 (13) : 1717 - 1727
[27] WRM-1 activates the LIT-1 protein kinase to transduce anterior posterior polarity signals in C-elegans
Rocheleau, CE
Yasuda, J
Shin, TH
Lin, RL
Sawa, H
Okano, H
Priess, JR
Davis, RJ
Mello, CC
[J]. CELL, 1999, 97 (06) : 717 - 726
[28] Wnt signaling and an APC-related gene specify endoderm in early C-elegans embryos
Rocheleau, CE
Downs, WD
Lin, RL
Wittmann, C
Bei, YX
Cha, YH
Ali, M
Priess, JR
Mello, CC
[J]. CELL, 1997, 90 (04) : 707 - 716
[29] LOCALIZATION AND SEGREGATION OF LINEAGE-SPECIFIC CLEAVAGE POTENTIAL IN EMBRYOS OF CAENORHABDITIS-ELEGANS
SCHIERENBERG, E
[J]. ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY, 1988, 197 (05): : 282 - 293
[30] Wnt pathway components orient a mitotic spindle in the early Caenorhabditis elegans embryo without requiring gene transcription in the responding cell
Schlesinger, A
Shelton, CA
Maloof, JN
Meneghini, M
Bowerman, B
[J]. GENES & DEVELOPMENT, 1999, 13 (15) : 2028 - 2038

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