SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C-elegans embryos

被引:124
作者
Bei, YX
Hogan, J
Berkowitz, LA
Soto, M
Rocheleau, CE
Pang, KM
Collins, J
Mello, CC [1 ]
机构
[1] Howard Hughes Med Inst, Coconut Grove, FL 33133 USA
[2] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA
[3] Univ New Hampshire, Dept Biochem & Mol Biol, Durham, NH 03824 USA
[4] Univ Tulsa, Dept Biol Sci, Tulsa, OK 74104 USA
关键词
D O I
10.1016/S1534-5807(02)00185-5
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In early C. elegans embryos, signaling between a posterior blastomere, P2, and a ventral blastomere, EMS, specifies endoderm and orients the division axis of the EMS cell. Although Wnt signaling contributes to this polarizing interaction, no mutants identified to date abolish P2/EMS signaling. Here, we show that two tyrosine kinase-related genes, src-1 and mes-1, are required for the accumulation of phosphotyrosine between P2 and EMS. Moreover, src-1 and mes-1 mutants strongly enhance endoderm and EMS spindle rotation defects associated with Wnt pathway mutants. SRC-1 and MES-1 signal bidirectionally to control cell fate and division orientation in both EMS and P2. Our findings suggest that Wnt and Src signaling function in parallel to control developmental outcomes within a single responding cell.
引用
收藏
页码:113 / 125
页数:13
相关论文
共 43 条
  • [21] Adherens junctions inhibit asymmetric division in the Drosophila epithelium
    Lu, BW
    Roegiers, F
    Jan, LY
    Jan, YN
    [J]. NATURE, 2001, 409 (6819) : 522 - 525
  • [22] McEwen DG, 2000, DEVELOPMENT, V127, P3607
  • [23] MAP kinase and Wnt pathways converge to downregulate an HMG-domain repressor in Caenorhabditis elegans
    Meneghini, MD
    Ishitani, T
    Carter, JC
    Hisamoto, N
    Ninomiya-Tsuji, J
    Thorpe, CJ
    Hamill, DR
    Matsumoto, K
    Bowerman, B
    [J]. NATURE, 1999, 399 (6738) : 793 - 797
  • [24] Papkoff J, 1997, J BIOL CHEM, V272, P4536
  • [25] Cancer - Wnt signaling in oncogenesis and embryogenesis - a look outside the nucleus
    Peifer, M
    Polakis, P
    [J]. SCIENCE, 2000, 287 (5458) : 1606 - 1609
  • [26] Drosophila Jun kinase regulates expression of decapentaplegic via the ETS-domain protein Aop and AP-1 transcription factor DJun during dorsal closure
    RiesgoEscovar, JR
    Hafen, E
    [J]. GENES & DEVELOPMENT, 1997, 11 (13) : 1717 - 1727
  • [27] WRM-1 activates the LIT-1 protein kinase to transduce anterior posterior polarity signals in C-elegans
    Rocheleau, CE
    Yasuda, J
    Shin, TH
    Lin, RL
    Sawa, H
    Okano, H
    Priess, JR
    Davis, RJ
    Mello, CC
    [J]. CELL, 1999, 97 (06) : 717 - 726
  • [28] Wnt signaling and an APC-related gene specify endoderm in early C-elegans embryos
    Rocheleau, CE
    Downs, WD
    Lin, RL
    Wittmann, C
    Bei, YX
    Cha, YH
    Ali, M
    Priess, JR
    Mello, CC
    [J]. CELL, 1997, 90 (04) : 707 - 716
  • [29] LOCALIZATION AND SEGREGATION OF LINEAGE-SPECIFIC CLEAVAGE POTENTIAL IN EMBRYOS OF CAENORHABDITIS-ELEGANS
    SCHIERENBERG, E
    [J]. ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY, 1988, 197 (05): : 282 - 293
  • [30] Wnt pathway components orient a mitotic spindle in the early Caenorhabditis elegans embryo without requiring gene transcription in the responding cell
    Schlesinger, A
    Shelton, CA
    Maloof, JN
    Meneghini, M
    Bowerman, B
    [J]. GENES & DEVELOPMENT, 1999, 13 (15) : 2028 - 2038