The role of single-stranded DNA and polymerase α in establishing the ATR, Hus1 DNA replication checkpoint

被引：96

作者：

You, ZZ ^{[1
]}

Kong, L ^{[1
]}

Newport, J ^{[1
]}

机构：

[1] Univ Calif San Diego, Div Biol, La Jolla, CA 92093 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2002年 / 277卷 / 30期

关键词：

D O I：

10.1074/jbc.M204120200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a nucleus-free DNA replication system we have investigated the roles of Xenopus ATR (YATR) and Hus1 (Xhus1) as the DNA replication checkpoint sensors. Like XATR, Xhus1 is required for the checkpoint-dependent phosphorylation of Xchk1 and associates with chromatin in an initiation-dependent manner. While removal of replication protein A inhibits chromatin association of both XATR and Xhus1, removal of polymerase a only disrupts chromatin association of Xhus1. In addition, chromatin association of XATR and Xhus1 are independent of each other. Finally, like XATR, Xhus1 associates with chromatin in unperturbed S phase and dissociates from chromatin following completion of DNA replication.

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页码：27088 / 27093

页数：6

相关论文

共 46 条

[1] The Schizosaccharomyces pombe rad3 checkpoint gene [J].

Bentley, NJ ;

Holtzman, DA ;

Flaggs, G ;

Keegan, KS ;

DeMaggio, A ;

Ford, JC ;

Hoekstra, M ;

Carr, AM .

EMBO JOURNAL, 1996, 15 (23) :6641-6651

[2]

Brown EJ, 2000, GENE DEV, V14, P397

[3] Reconstitution and molecular analysis of the hRad9-hHus1-hRad1 (9-1-1) DNA damage responsive checkpoint complex [J].

Burtelow, MA ;

Roos-Mattjus, PMK ;

Rauen, M ;

Babendure, JR ;

Karnitz, LM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (28) :25903-25909

[4] Characterization of Schizosaccharomyces pombe Hus1:: a PCNA-related protein that associates with Rad1 and Rad9 [J].

Caspari, T ;

Dahlen, M ;

Kanter-Smoler, G ;

Lindsay, HD ;

Hofmann, K ;

Papadimitriou, K ;

Sunnerhagen, P ;

Carr, AM .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (04) :1254-1262

[5] cDNA cloning and gene mapping of a candidate human cell cycle checkpoint protein [J].

Cimprich, KA ;

Shin, TB ;

Keith, CT ;

Schreiber, SL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (07) :2850-2855

[6] CDC2 REGULATORY FACTORS [J].

COLEMAN, TR ;

DUNPHY, WG .

CURRENT OPINION IN CELL BIOLOGY, 1994, 6 (06) :877-882

[7] ATR and ATRIP: Partners in checkpoint signaling [J].

Cortez, D ;

Guntuku, S ;

Qin, J ;

Elledge, SJ .

SCIENCE, 2001, 294 (5547) :1713-1716

[8] Targeted disruption of the cell-cycle checkpoint gene ATR leads to early embryonic lethality in mice [J].

de Klein, A ;

Muijtjens, M ;

van Os, R ;

Verhoeven, Y ;

Smit, B ;

Carr, AM ;

Lehmann, AR ;

Hoeijmakers, JHJ .

CURRENT BIOLOGY, 2000, 10 (08) :479-482

[9] Cell cycle checkpoints: Preventing an identity crisis [J].

Elledge, SJ .

SCIENCE, 1996, 274 (5293) :1664-1672

[10] Cdc25 inhibited in vivo and in vitro by checkpoint kinases Cds1 and Chk1 [J].

Furnari, B ;

Blasina, A ;

Boddy, MN ;

McGowan, CH ;

Russell, P .

MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (04) :833-845

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