Effect of recombinant human gonadotrophins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro

被引:87
作者
Anderiesz, C
Ferraretti, AP
Magli, C
Fiorentino, A
Fortini, D
Gianaroli, L
Jones, GM
Trounson, AO
机构
[1] Monash Med Ctr, Monash Inst Reprod & Dev, Clayton, Vic 3168, Australia
[2] Soc Italinana Studi Med Reprod SRL, Reprod Med Unit, Bologna, Italy
关键词
embryonic development; immature oocytes; in-vitro maturation; oocyte proteins; recombinant gonadotrophins;
D O I
10.1093/humrep/15.5.1140
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The response of murine, bovine and human oocytes to pure recombinant preparations of human follicle stimulating hormone (rFSH) and luteinizing hormone (rLH) for meiotic maturation and subsequent developmental competence in vitro were examined in the present experiments. Maturation of immature bovine oocytes to the metaphase II stage was significantly increased by the addition of 1 IU/ml of rFSH in combination with either 1 IU/ml rLH or 10 IU/ml rLH. Similarly, embryonic development to the blastocyst stage was improved in bovine oocytes treated with a 1:10 combination of rFSH:rLH. However, no significant difference was observed in the number of inner cell mass or trophectoderm cells of the resulting blastocysts, Although the increased maturation to metaphase II was not significant, human embryonic developmental competence was improved by maturing oocytes in the presence of a 1:10 ratio of rFSH:rLH as only those oocytes exposed to a 1:10 ratio of rFSH:rLH during maturation showed normal cleavage patterns beyond day 2. In addition, 1 IU/ml rFSH and 1 IU/ml rLH increased the expression of oocyte proteins in human oocytes. The inclusion of recombinant gonadotrophins, either singly or in combination, had no significant effect on the maturation, fertilization or embryonic development of in-vitro matured mouse oocytes. These data provide support for the responsiveness of human and bovine oocytes to gonadotrophins in vitro and the need to consider variations in the relative concentrations for optimization of oocyte developmental competence.
引用
收藏
页码:1140 / 1148
页数:9
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