Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A

被引：109

作者：

Gruswitz, Franz ^{[1
]}

O'Connell, Joseph, III ^{[1
]}

Stroud, Robert M. ^{[1
]}

机构：

[1] Univ Calif San Francisco, Sch Med, Dept Biochem & Biophys, San Francisco, CA 94143 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 01期

关键词：

membrane; regulation; structure; allosteric; dinucleotide;

D O I：

10.1073/pnas.0609796104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ammonia conductance is highly regulated. A P-parallel to signal transduction protein, GInK, is the final regulator of transmembrane ammonia conductance by the ammonia channel AmtB in Escherichia coli. The complex formed between AmtB and inhibitory GInK at 1.96-angstrom resolution shows that the trimeric channel is blocked directly by GInK and how, in response to intracellular nitrogen status, the ability of GInK to block the channel is regulated by uridylylation/ deuridylylation at Y51. ATP and Mg2+ augment the interaction of GlnK. The hydrolyzed product, adenosine 5'-diphosphate orients the surface of GInK for AmtB blockade. 2-Oxoglutarate diminishes AmtB/GInK association, and sites for 2-oxoglutarate are evaluated.

引用

页码：42 / 47

页数：6

共 25 条

[1] Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus [J].

Andrade, SLA ;

Dickmanns, A ;

Ficner, R ;

Einsle, O .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (42) :14994-14999

[2] Herbaspirillum seropedicae signal transduction protein PII is structurally similar to the enteric GlnK [J].

Benelli, EM ;

Buck, M ;

Polikarpov, I ;

de Souza, EM ;

Cruz, LM ;

Pedrosa, FO .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2002, 269 (13) :3296-3303

[3] MUTANT STRAINS (NIT) OF SALMONELLA-TYPHIMURIUM WITH A PLEIOTROPIC DEFECT IN NITROGEN-METABOLISM [J].

BROACH, J ;

NEUMANN, C ;

KUSTU, S .

JOURNAL OF BACTERIOLOGY, 1976, 128 (01) :86-98

[4] STRUCTURE OF THE ESCHERICHIA-COLI SIGNAL-TRANSDUCING PROTEIN P-II [J].

CHEAH, E ;

CARR, PD ;

SUFFOLK, PM ;

VASUDEVAN, SG ;

DIXON, NE ;

OLLIS, DL .

STRUCTURE, 1994, 2 (10) :981-990

[5] PIBASE: a comprehensive database of structurally defined protein interfaces [J].

Davis, FP ;

Sali, A .

BIOINFORMATICS, 2005, 21 (09) :1901-1907

[6] In vitro analysis of the Escherichia coli AmtB-GlnK complex reveals a stoichiometric interaction and sensitivity to ATP and 2-oxoglutarate [J].

Durand, Anne ;

Merrick, Mike .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (40) :29558-29567

[7] Unique mechanistic features of post-translational regulation of glutamine synthetase activity in Methanosarcina mazei strain Go1 in response to nitrogen availability [J].

Ehlers, C ;

Weidenbach, K ;

Veit, K ;

Forchhammer, K ;

Schmitz, RA .

MOLECULAR MICROBIOLOGY, 2005, 55 (06) :1841-1854

[8] Structure-function relationships of glutamine synthetases [J].

Eisenberg, D ;

Gill, HS ;

Pfluegl, GMU ;

Rotstein, SH .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1477 (1-2) :122-145

[9] DOCK 4.0: Search strategies for automated molecular docking of flexible molecule databases [J].

Ewing, TJA ;

Makino, S ;

Skillman, AG ;

Kuntz, ID .

JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN, 2001, 15 (05) :411-428

[10] Ammonium sensing in Escherichia coli -: Role of the ammonium transporter AmtB and AmtB-GlnK complex formation [J].

Javelle, A ;

Severi, E ;

Thornton, J ;

Merrick, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (10) :8530-8538

← 1 2 3 →