NADPH oxidase contributes to angiotensin II signaling in the nucleus tractus solitarius

被引:150
作者
Wang, G
Anrather, J
Huang, J
Speth, RC
Pickel, VM
Iadecola, C
机构
[1] Cornell Univ, Weill Med Coll, Div Neurobiol, Dept Neurol & Neurosci, New York, NY 10021 USA
[2] Univ Mississippi, Sch Pharm, Dept Pharmacol, University, MS 38677 USA
关键词
nucleus of the solitary tract; angiotensin II; NADPH oxidase; patch clamp; reactive oxygen species; voltage-gated Ca2+ channels;
D O I
10.1523/JNEUROSCI.1176-04.2004
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Angiotensin II (AngII), acting through angiotensin type 1 (AT(1)) receptors, exerts powerful effects on central autonomic networks regulating cardiovascular homeostasis and fluid balance; however, the mechanisms of AngII signaling in functionally defined central autonomic neurons have not been fully elucidated. In vascular cells, reactive oxygen species (ROS) generated by the enzyme NADPH oxidase play a major role in AngII signaling. Thus, we sought to determine whether NADPH oxidase is present in central autonomic neurons and, if so, whether NADPH oxidase-derived ROS are involved in the effects of AngII on these neurons. The present studies focused on the intermediate dorsomedial nucleus of the solitary tract (dmNTS) because this region receives autonomic afferents via the vagus nerve and is an important site of AngII actions. Using double-label immunoelectron microscopy, we found that the essential NADPH oxidase subunit gp91(phox) is present in somatodendric and axonal profiles containing AT(1) receptors. The gp91(phox)-labeled dendrites received inputs from large axon terminals resembling vagal afferents. In parallel experiments using patch clamp of dissociated NTS neurons anterogradely labeled via the vagus, we found that AngII potentiates the L-type Ca2+ currents, an effect mediated by AT(1) receptors and abolished by the ROS scavenger Mn(III) tetrakis (4-benzoic acid) porphyrin chloride. The NADPH oxidase assembly inhibitor apocynin and the peptide inhibitor gp91(phox) docking sequence, but not its scrambled version, also blocked the potentiation. The results provide evidence that NADPH oxidase-derived ROS are involved in the effects of AngII on Ca2+ influx in NTS neurons receiving vagal afferents and support the notion that ROS are important signaling molecules in central autonomic networks.
引用
收藏
页码:5516 / 5524
页数:9
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