Disulfide cross-linking of subunits F1-γ and F0I-PVP(b) results in asymmetric effects on proton translocation in the mitochondrial ATP synthase.

被引：7

作者：

Gaballo, A

Zanotti, F

Raho, G

Papa, S

机构：

[1] Univ Bari, Dept Med Biochem & Biol, I-70125 Bari, Italy

[2] Univ Bari, CNR, Ctr Study Mitochondria & Energy Metab, Bari, Italy

来源：

FEBS LETTERS | 1999年 / 463卷 / 1-2期

关键词：

H-perpendicular to-ATP synthase; F-0; F-1; F0I-PVP(b) subunit; F-1-gamma subunit; H+ translocation;

D O I：

10.1016/S0014-5793(99)01593-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A study is presented on the effect of diamide-induced disulfide cross-linking of F-1-gamma and F0I-PVP(b) subunits on proton translocation in the mitochondrial ATP synthase, The results show that, upon cross-linking of these subunits, whilst proton translocation from the A side to the B F-1 side is markedly accelerated with decoupling of oxidative phosphorylation, proton translocation in the reverse direction, driven by either ATP hydrolysis or a diffusion potential, is unaffected. These observations reveal further peculiarities of the mechanism of energy transfer in the ATP synthase of coupling membranes. (C) 1999 Federation of European Biochemical Societies.

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页码：7 / 11

页数：5

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