A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells

被引：225

作者：

Chen, Peng R. ^{[2
,3
]}

Groff, Dan ^{[2
,3
]}

Guo, Jiantao ^{[2
,3
]}

Ou, Weijia ^{[1
]}

Cellitti, Susan ^{[1
]}

Geierstanger, Bernhard H. ^{[1
]}

Schultz, Peter G. ^{[1
,2
,3
]}

机构：

[1] Genom Inst Novartis Res Fdn, San Diego, CA 92121 USA

[2] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 22期

关键词：

gene expression; nonnatural amino acids; photocaged lysine; tRNA; TRANSFER-RNA SYNTHETASE; GENETIC-CODE; PROTEINS; PHOSPHORYLATION; LYSINE;

D O I：

10.1002/anie.200900683

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：4052 / 4055

页数：4

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