Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes

被引：45

作者：

Liu, Jifeng

Su, Bin

Lagger, Gregoire

Tacchini, Philippe

Girault, Hubert H. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Electrochimie Phys & Analyt, Stn 6, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, EDEL Therapeut SA, PSE B, CH-1015 Lausanne, Switzerland

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 19期

关键词：

D O I：

10.1021/ac0608624

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes were developed. The carbon ink was doped with TiO2 nanoparticles, onto which double-strand DNA was adsorbed. A redox mediator, namely, tris-2,2'-bipyridine ruthenium(II) [Ru(bpy)(3)(2+)] was electrooxidized on the electrode surface to subsequently oxidize both the adsorbed ds-DNA and the antioxidants in solution. The resulting oxidation damage of the adsorbed dsDNA was then detected by square wave voltammetry in a second solution containing only Ru(bpy)(3)Cl-2 at a low concentration (mu M). A kinetic model was developed to study the protecting role of antioxidants in aqueous solutions. The electrochemical sensor has been applied to evaluate the redox antioxidant capacity of different molecules.

引用

页码：6879 / 6884

页数：6

共 47 条

[1]

Antolovich M, 2002, ANALYST, V127, P183, DOI 10.1039/b009171p

[2] Electrochemical detection of gene expression in tumor samples: Overexpression of Rak nuclear tyrosine kinase [J].