Synthesis of oligodeoxynucleotides containing 2-substituted guanine derivatives using 2-fluoro-2'-deoxyinosine as common nucleoside precursor.

被引:21
作者
Diaz, AR [1 ]
Eritja, R [1 ]
Garcia, RG [1 ]
机构
[1] EUROPEAN MOL BIOL LAB,D-69117 HEIDELBERG,GERMANY
来源
NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 10-11期
关键词
D O I
10.1080/07328319708002554
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oligonucleotides containing 2-substituted guanine derivatives with double-helix stabilizing molecules such as spermine, spermidine and propylimidazole have been prepared using protected 2-fluoro-2'-deoxyinosine phosphoramidite and two different protective strategies: the p-nitrophenylethyl (NPE) and the t-butylphenoxyacetyl groups. Melting studies show a large increase on the melting temperatures of duplexes containing these 2-substituted guanine derivatives.
引用
收藏
页码:2035 / 2051
页数:17
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