A novel Rho GTPase-activating-protein interacts with Gem, a member of the Ras superfamily of GTPases

被引:45
作者
Aresta, S [1 ]
De Tand-Heim, MF [1 ]
Béranger, F [1 ]
De Gunzburg, J [1 ]
机构
[1] Inst Curie, INSERM, U528, Sect Rech, F-75248 Paris 05, France
关键词
cytoskeleton; in vivo activity; RhoA;
D O I
10.1042/BJ20020829
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gem is a Ras-related protein whose expression is induced in several cell types upon activation by extracellular stimuli. With the aim of isolating the cellular partners of Gem that mediate its biological activity we performed a yeast two-hybrid screen and identified a novel protein of 970 amino acids, Gmip, that interacts with Gem through its N-terminal half, and presents a cysteine-rich domain followed by a Rho GTPase-activating protein (RhoGAP) domain in its C-terminal half. The RhoGAP domain of Gmip stimulates in vitro the GTPase activity of RhoA, but is inactive towards other Rho family proteins such as Rac1 and Cdc42; it is also specific for RhoA in vivo. The same is true for the full-length protein, which is furthermore able to downregulate RhoA-dependent stress fibres in Ref-52 rat fibroblasts. These findings suggest that the signalling pathways controlled by two proteins of the Ras superfamily, RhoA and Gem, are linked via the action of the RhoGAP protein Gmip (Gem-interacting protein).
引用
收藏
页码:57 / 65
页数:9
相关论文
共 39 条
  • [11] Fritz G, 1999, INT J CANCER, V81, P682, DOI 10.1002/(SICI)1097-0215(19990531)81:5<682::AID-IJC2>3.0.CO
  • [12] 2-B
  • [13] A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion
    Fu, YX
    Galán, JE
    [J]. NATURE, 1999, 401 (6750) : 293 - 297
  • [14] Molecular dissection of the Rho-associated protein kinase (p160ROCK)-regulated neurite remodeling in neuroblastoma N1E-115 cells
    Hirose, M
    Ishizaki, T
    Watanabe, N
    Uehata, M
    Kranenburg, O
    Moolenaar, WH
    Matsumura, F
    Maekawa, M
    Bito, H
    Narumiya, S
    [J]. JOURNAL OF CELL BIOLOGY, 1998, 141 (07) : 1625 - 1636
  • [15] INHIBITION OF LYSOPHOSPHATIDATE-INDUCED AND THROMBIN-INDUCED NEURITE RETRACTION AND NEURONAL CELL ROUNDING BY ADP-RIBOSYLATION OF THE SMALL GTP-BINDING PROTEIN-RHO
    JALINK, K
    VANCORVEN, EJ
    HENGEVELD, T
    MORII, N
    NARUMIYA, S
    MOOLENAAR, WH
    [J]. JOURNAL OF CELL BIOLOGY, 1994, 126 (03) : 801 - 810
  • [16] Regulation of the cytoskeleton and cell adhesion by the Rho family GTPases in mammalian cells
    Kaibuchi, K
    Kuroda, S
    Amano, M
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 : 459 - 486
  • [17] KOZAK M, 1991, J BIOL CHEM, V266, P19867
  • [18] Kozma R, 1996, MOL CELL BIOL, V16, P5069
  • [19] GAPS FOR RHO-RELATED GTPASES
    LAMARCHE, N
    HALL, A
    [J]. TRENDS IN GENETICS, 1994, 10 (12) : 436 - 440
  • [20] The Gem GTP-binding protein promotes morphological differentiation in neuroblastoma
    Leone, A
    Mitsiades, N
    Ward, Y
    Spinelli, B
    Poulaki, V
    Tsokos, M
    Kelly, K
    [J]. ONCOGENE, 2001, 20 (25) : 3217 - 3225