PGC7 suppresses TET3 for protecting DNA methylation

被引：53

作者：

Bian, Chunjing ^{[1
]}

Yu, Xiaochun ^{[1
]}

机构：

[1] Univ Michigan, Dept Internal Med, Div Mol Med & Genet, Sch Med, Ann Arbor, MI 48109 USA

来源：

NUCLEIC ACIDS RESEARCH | 2014年 / 42卷 / 05期

关键词：

GENE-EXPRESSION; 5-HYDROXYMETHYLCYTOSINE; 5-METHYLCYTOSINE; PROTEINS; GENOME; DEMETHYLATION; DIOXYGENASE; MECHANISMS; CONVERSION; DYNAMICS;

D O I：

10.1093/nar/gkt1261

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ten-eleven translocation (TET) family enzymes convert 5-methylcytosine to 5-hydroxylmethylcytosine. However, the molecular mechanism that regulates this biological process is not clear. Here, we show the evidence that PGC7 (also known as Dppa3 or Stella) interacts with TET2 and TET3 both in vitro and in vivo to suppress the enzymatic activity of TET2 and TET3. Moreover, lacking PGC7 induces the loss of DNA methylation at imprinting loci. Genome-wide analysis of PGC7 reveals a consensus DNA motif that is recognized by PGC7. The CpG islands surrounding the PGC7-binding motifs are hypermethylated. Taken together, our study demonstrates a molecular mechanism by which PGC7 protects DNA methylation from TET family enzyme-dependent oxidation.

引用

页码：2893 / 2905

页数：13

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