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Curvature-dependent lateral distribution of raft markers in the human erythrocyte membrane
被引:61
作者:
Hagerstrand, Henry
[1
]
Mrowczynska, Lucyna
Salzer, Ulrich
Prohaska, Rainer
Michelsen, Kimmo A.
Kralj-Iglic, Veronika
Iglic, Ales
机构:
[1] Abo Akad Univ, Dept Biol, FIN-20520 Turku, Finland
[2] Adam Mickiewicz Univ Poznan, Dept Cell Biol, Poznan, Poland
[3] Med Univ Vienna, Dept Biochem Med, Max F Perutz Labs, Vienna, Austria
[4] Univ Ljubljana, Phys Lab, Fac Elect Engn, Ljubljana, Slovenia
[5] Univ Ljubljana, Inst Biophys, Fac Med, Ljubljana, Slovenia
基金:
奥地利科学基金会;
芬兰科学院;
关键词:
erythrocyte;
membrane microdomain;
raft;
membrane curvature;
intrinsic curvature;
amphiphile;
calcium ionophore A23187;
D O I:
10.1080/09687860600682536
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The distribution of raft markers in curved membrane exvaginations and invaginations, induced in human erythrocytes by amphiphile-treatment or increased cytosolic calcium level, was studied by fluorescence microscopy. Cholera toxin subunit B and antibodies were used to detect raft components. Ganglioside GM1 was enriched in membrane exvaginations (spiculae) induced by cytosolic calcium and amphiphiles. Stomatin and the cytosolic proteins synexin and sorcin were enriched in spiculae when induced by cytosolic calcium, but not in spiculae induced by amphiphiles. No enrichment of flotillin-1 was detected in spiculae. Analyses of the relative protein content of released exovesicles were in line with the microscopic observations. In invaginations induced by amphiphiles, the enrichment of ganglioside GM1, but not of the integral membrane proteins flotillin-1 and stomatin, was observed. Based on the experimental results and theoretical considerations we suggest that membrane skeleton-detached, laterally mobile rafts may sort into curved or flat membrane regions dependent on their intrinsic molecular shape and/or direct interactions between the raft elements.
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页码:277 / 288
页数:12
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