Peptide separation by Hydrophilic-Interaction Chromatography: a review

被引：218

作者：

Yoshida, T ^{[1
]}

机构：

[1] Tosoh Anal & Res Ctr, Ayase, Kanagawa 2521123, Japan

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2004年 / 60卷 / 03期

关键词：

Hydrophilic-Interaction Chromatography; Reversed-Phase Liquid Chromatography; selectivity; peptides; amino acids; retention parameter;

D O I：

10.1016/j.jbbm.2004.01.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent developments in the separation of peptides by high-performance liquid chromatography (HPLC) using polar sorbents with less polar eluents are summarized in this review. This separation mode is now commonly referred to as Hydrophilic-Interaction Chromatography (HILIC). The retention mechanism and chromatographic behavior of polar solutes under HILIC conditions are studied on TSKgel Amide-80 columns, which consist of carbamoyl groups bonded to a silica gel matrix, using a mixture of acetonitrile (MeCN)-water containing 0.1% trifluoroacetic acid (TFA). Some applications are given in peptide field using Hydrophilic-Interaction Chromatography. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：265 / 280

页数：16

共 43 条

[1] HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS [J].