Recent progress in carbohydrate separation by high-performance liquid chromatography based on hydrophilic interaction

This article surveys recent developments in the separation and analysis of carbohydrates by high-performance liquid chromatography, in adsorption or partition modes, on polar sorbents with less polar eluents, a technique that is now termed hydrophilic interaction chromatography. A variety of chromatographic methods are included under this generic heading, the most important being adsorption chromatography on silica and partition chromatography on silica-based sorbents bearing bonded polar phases. Examples are given of the applications of these stationary phases, as well as the newer polymer-based polar sorbents, in high-performance liquid chromatography of carbohydrates and their derivatives.