Different bindings of the minor groove ligands DAPI and Hoechst 33258 to multimers of the curved (CA(4)T(4)G) and noncurved (CT(4)A(4)G) DNA sequences

被引：13

作者：

Caneva, RC

DeSimoni, A

Mayol, L

Rossetti, L

Savino, M

机构：

[1] UNIV ROMA LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOL,I-00185 ROME,ITALY

[2] UNIV ROMA LA SAPIENZA,IST PASTEUR,FDN CENCI BOLOGNETTI,I-00185 ROME,ITALY

[3] UNIV NAPLES,DIPARTIMENTO CHIM SOSTANZE NAT,I-80131 NAPLES,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1353卷 / 02期

关键词：

DNA curvature; CA(4)T(4)G; CT(4)A(4)G; minor groove; DAPI; Hoechst; 33258;

D O I：

10.1016/S0167-4781(97)00091-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Equilibrium binding properties of DAPI and Hoechst for prototype curved (CA(4)T(4)G)(n) and straight (CT(4)A(4)G)(n) DNAs were derived from fluorescence intensity. Both decamers display a single strong binding site for each ligand. Hoechst binds seven times stronger to curved than to straight DNA. An additional cooperative, much weaker binding site is found for DAPI. (C) 1997 Elsevier Science B.V.

引用

收藏

页码：93 / 97

页数：5

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