Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens

被引：89

作者：

Chattopadhyay, S

Patra, R

Ramamurthy, T

Chowdhury, A

Santra, A

Dhali, GK

Bhattacharya, SK

Berg, DE

Nair, GB

Mukhopadhyay, AK

机构：

[1] Natl Inst Cholera & Enter Dis, Scheme XM, Kolkata 700010, W Bengal, India

[2] Postgrad Inst Med Educ & Res, Dept Gastroenterol, Kolkata, W Bengal, India

[3] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

[4] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 06期

关键词：

D O I：

10.1128/JCM.42.6.2821-2824.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.

引用

收藏

页码：2821 / 2824

页数：4

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