Polymyxin B binds to anandamide and inhibits its cytotoxic effect

被引：90

作者：

Wang, Y

Liu, Y

Sarker, KP

Nakashima, M

Serizawa, T

Kishida, A

Akashi, M

Nakata, M

Kitajima, I

Maruyama, I

机构：

[1] Kagoshima Univ, Fac Med, Dept Lab & Mol Med, Kagoshima 8908520, Japan

[2] Kagoshima Univ, Fac Engn, Dept Chem Engn & Appl Chem, Kagoshima 8908520, Japan

来源：

FEBS LETTERS | 2000年 / 470卷 / 02期

关键词：

cannabinoid; anandamide; polymyxin B; endotoxin shock; surface plasmon resonance;

D O I：

10.1016/S0014-5793(00)01313-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to he a paracrine contributor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)-immobilized beads column and eluted with ethanol, We confirmed the direct binding of PMB to ANA by using surface plasmon resonance. The adsorption of ANA by PMB may abolish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin shock. (C) 2000 Federation of European Biochemical Societies.

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页码：151 / 155

页数：5

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