Molecular identification of methanogenic archaea from sheep in Queensland, Australia reveal more uncultured novel archaea

被引：85

作者：

Wright, ADG ^{[1
]}

Toovey, AF ^{[1
]}

Pimm, CL ^{[1
]}

机构：

[1] CSIRO Livestock Ind, Ctr Environm & Life Sci, Wembley, WA 6913, Australia

来源：

ANAEROBE | 2006年 / 12卷 / 03期

关键词：

methanogens; Methanobrevibacter; rumen ecology; ruminants; sheep;

D O I：

10.1016/j.anaerobe.2006.02.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Molecular diversity of rumen methanogens in sheep in Queensland, Australia was investigated using 16S rRNA gene libraries prepared from pooled rumen contents from nine merino sheep. A total of 78 clones were identified revealing 26 different sequences. Of these 26 sequences, eight sequences (15 clones) were 95-100% similar to cultivated methanogens belonging to the orders Methanobacteriales and Methanomicrobiales, and the remaining IS phylotypes (63 clones) were 72-75% similar to Thermoplasma acidophilum and Thermoplasma volcanium. These unique sequences clustered within a distinct and strongly supported (100% bootstrap support) phylogenetic group, exclusively composed of sequences from uncharacterized archaea from very diverse anaerobic environments. Members of this unique group that were previously considered atypical for the rumen environment were the predominant clones. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：134 / 139

页数：6

共 34 条

[21] Phylogenetic analysis of archaea in three fractions of cow rumen based on the 16S rDNA sequence [J].

Shin, EC ;

Choi, BR ;

Lim, WJ ;

Hong, SY ;

An, CL ;

Cho, KM ;

Kim, YK ;

An, JM ;

Kang, JM ;

Lee, SS ;

Kim, H ;

Yun, HD .

ANAEROBE, 2004, 10 (06) :313-319

[22]

Skillman LC, 2006, LETT APPL MICROBIOL, V42, P222, DOI [10.1111/j.1472-765X.2005.01833.x, 10.1111/j.1472-765X.2005.01833.X]

[23] 16S ribosomal DNA-directed PCR primers for ruminal methanogens and identification of methanogens colonising young lambs [J].

Skillman, LC ;

Evans, PN ;

Naylor, GE ;

Morvan, B ;

Jarvis, GN ;

Joblin, KN .

ANAEROBE, 2004, 10 (05) :277-285

[24] Characterisation of the microbial diversity in a pig manure storage pit using small subunit rDNA sequence analysis [J].

Snell-Castro, R ;

Godon, JJ ;

Delgenès, JP ;

Dabert, P .

FEMS MICROBIOLOGY ECOLOGY, 2005, 52 (02) :229-242

[25] Phylogenetic analysis of archaeal 16S rRNA libraries from the rumen suggests the existence of a novel group of archaea not associated with known methanogens [J].

Tajima, K ;

Nagamine, T ;

Matsui, H ;

Nakamura, M ;

Aminov, RI .

FEMS MICROBIOLOGY LETTERS, 2001, 200 (01) :67-72

[26] Rumen bacterial community transition during adaptation to high-grain diet [J].

Tajima, K ;

Arai, S ;

Ogata, K ;

Nagamine, T ;

Matsui, H ;

Nakamura, M ;

Aminov, RI ;

Benno, Y .

ANAEROBE, 2000, 6 (05) :273-284

[27] Phylogenetic study of methanogens associated with rumen ciliates [J].

Tokura, M ;

Chagan, I ;

Ushida, K ;

Kojima, Y .

CURRENT MICROBIOLOGY, 1999, 39 (03) :123-128

[28] Phylogenetic comparison of methanogen diversity in different wetland soils [J].

Utsumi, M ;

Belova, SE ;

King, GM ;

Uchiyama, H .

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2003, 49 (02) :75-83

[29] Phylogenetic analysis of methanogens from the bovine rumen [J].

Whitford M.F. ;

Teather R.M. ;

Forster R.J. .

BMC Microbiology, 1 (1) :1-5

[30]

Wolin M.J., 1997, RUMEN MICROBIAL ECOS, V2nd, P467, DOI DOI 10.1007/978-94-009-1453-7_11

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